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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
- Other assay [1]
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- Product number
- PA5-20085 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Btk Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is U937 cell lysate. PA5-20085 can be used with blocking peptide PEP-0203.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Safety, Tolerability, Pharmacokinetics, Target Occupancy, and Concentration-QT Analysis of the Novel BTK Inhibitor Evobrutinib in Healthy Volunteers.
Becker A, Martin EC, Mitchell DY, Grenningloh R, Bender AT, Laurent J, Mackenzie H, Johne A
Clinical and translational science 2020 Mar;13(2):325-336
Clinical and translational science 2020 Mar;13(2):325-336
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Btk was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-Btk Polyclonal Antibody (Product # PA5-20085) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma Membrane and Cytoplasmic localization. Panel e represents negative control, Jurkat cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of BTK in Daudi cells with Btk Polyclonal Antibody (Product # PA5-20085) at 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of BTK in Daudi cells with Btk Polyclonal Antibody (Product # PA5-20085) at 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Arithmetic mean (SD) of % Bruton's tyrosine kinase (BTK) receptor occupancy* vs. time after administration of evobrutinib for ( a ) single dosing and ( b ) multiple dosing. *% BTK occupancy = (1-(BTK F /BTK T )/(BTK F pre-dose/BTK T pre-dose))*100. BTK T , total Bruton's tyrosine kinase; BTK F , free Bruton's tyrosine kinase.
