Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB7205 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-BTK (Y223) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Phospho-BTK (Y223) in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 720101
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-BTK (Y223) by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate for 30 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-BTK (Y223) Monoclonal Antibody (Catalog # MAB7205) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-BTK (Y223) at approximately 80 kDa (as indicated). For additional reference, duplicate samples were probed with 0.05 µg/mL of Mouse Anti-Human BTK Monoclonal Antibody (lower panel, Catalog # MAB5807) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Phospho-BTK (Y223) in Raji Human Cell Line. BTK phosphorylated at Y223 was detected in immersion fixed Raji human Burkitt's lymphoma cell line with (upper panel) and without (lower panel) pervanadate stimulation using Mouse Anti-Human Phospho-BTK (Y223) Monoclonal Antibody (Catalog # MAB7205) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.