Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB300-289 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-289, RRID:AB_2082167
- Product name
- Rabbit Polyclonal Collagen XXV alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This detects full-length CLAC-P and the secreted form of CLAC-P protein. It does not react with full-length or secreted forms of COLXIII protein or the COLXXIII protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Collagen XXV alpha 1 Antibody [NB300-289] - Stable HEK293 cell line expressing CLAC-P using NB300-289. Lane 1: full-length CLAC-P (lysate). Lane 2: secreted form of CLAC-P (media). Lane 3: COLXIII (lysate). Lane 4 degrees COLXIII (media). Lane 5: COLXXIII (lysate). Lane 6: COLXXIII (media).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen XXV Antibody [NB300-289] - Immunostain of the neocortex of Alzheimer's diseased brain sections (floating, fixed with 10% formalin for 24 hrs) using NB 300-289. At high magnification, NB 300-289 stains senile plaques.