PAB29363

antibody from Abnova Corporation

Targeting: LIG4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29363

Provider

Abnova Corporation

Product name

LIG4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human LIG4.

Antigen sequence

TYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFK
TKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYF
IDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEY
RYSWDCSPLSMFRRHTVYLDSYA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with LIG4 polyclonal antibody (Cat # PAB29363).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with LIG4 polyclonal antibody (Cat # PAB29363) at 1-4 ug/mL concentration shows positivity in plasma membrane, cytoplasm, focal adhesion sites and nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with LIG4 polyclonal antibody (Cat # PAB29363) shows moderate nuclear positivity in cells in seminiferus ducts at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)