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LearnNB100-473
antibody from Novus Biologicals
Targeting: MRE11
ATLD, MRE11A
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Functional assay Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [1]
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- Product number
- NB100-473 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-473, RRID:AB_10001780
- Product name
- Mouse Monoclonal Mre11 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Blockade of vascular endothelial growth factor receptor 2 inhibits intraplaque haemorrhage by normalization of plaque neovessels.
Ataxia telangiectasia mutated- and Rad3-related kinase drives both the early and the late DNA-damage response to the monofunctional antitumour alkylator S23906.
MRE11 complex links RECQ5 helicase to sites of DNA damage.
MRN complex function in the repair of chromosomal Rag-mediated DNA double-strand breaks.
MRE11 expression is impaired in gastric cancer with microsatellite instability.
Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability.
NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors.
Requirement of the MRN complex for ATM activation by DNA damage.
Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage.
NFBD1/KIAA0170 is a chromatin-associated protein involved in DNA damage signaling pathways.
Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks.
Distinct functions of Nijmegen breakage syndrome in ataxia telangiectasia mutated-dependent responses to DNA damage.
MDC1 is a mediator of the mammalian DNA damage checkpoint.
Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex.
UV-induced replication arrest in the xeroderma pigmentosum variant leads to DNA double-strand breaks, gamma -H2AX formation, and Mre11 relocalization.
Interaction of FANCD2 and NBS1 in the DNA damage response.
Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome.
Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome.
ATM-dependent phosphorylation of nibrin in response to radiation exposure.
Nijmegen breakage syndrome disease protein and MRE11 at PML nuclear bodies and meiotic telomeres.
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
de Vries MR, Parma L, Peters HAB, Schepers A, Hamming JF, Jukema JW, Goumans MJTH, Guo L, Finn AV, Virmani R, Ozaki CK, Quax PHA
Journal of internal medicine 2019 Jan;285(1):59-74
Journal of internal medicine 2019 Jan;285(1):59-74
Ataxia telangiectasia mutated- and Rad3-related kinase drives both the early and the late DNA-damage response to the monofunctional antitumour alkylator S23906.
Soares DG, Battistella A, Rocca CJ, Matuo R, Henriques JA, Larsen AK, Escargueil AE
The Biochemical journal 2011 Jul 1;437(1):63-73
The Biochemical journal 2011 Jul 1;437(1):63-73
MRE11 complex links RECQ5 helicase to sites of DNA damage.
Zheng L, Kanagaraj R, Mihaljevic B, Schwendener S, Sartori AA, Gerrits B, Shevelev I, Janscak P
Nucleic acids research 2009 May;37(8):2645-57
Nucleic acids research 2009 May;37(8):2645-57
MRN complex function in the repair of chromosomal Rag-mediated DNA double-strand breaks.
Helmink BA, Bredemeyer AL, Lee BS, Huang CY, Sharma GG, Walker LM, Bednarski JJ, Lee WL, Pandita TK, Bassing CH, Sleckman BP
The Journal of experimental medicine 2009 Mar 16;206(3):669-79
The Journal of experimental medicine 2009 Mar 16;206(3):669-79
MRE11 expression is impaired in gastric cancer with microsatellite instability.
Ottini L, Falchetti M, Saieva C, De Marco M, Masala G, Zanna I, Paglierani M, Giannini G, Gulino A, Nesi G, Mariani Costantini R, Palli D
Carcinogenesis 2004 Dec;25(12):2337-43
Carcinogenesis 2004 Dec;25(12):2337-43
Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability.
Giannini G, Rinaldi C, Ristori E, Ambrosini MI, Cerignoli F, Viel A, Bidoli E, Berni S, D'Amati G, Scambia G, Frati L, Screpanti I, Gulino A
Oncogene 2004 Apr 8;23(15):2640-7
Oncogene 2004 Apr 8;23(15):2640-7
NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors.
Xu X, Stern DF
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2003 Oct;17(13):1842-8
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2003 Oct;17(13):1842-8
Requirement of the MRN complex for ATM activation by DNA damage.
Uziel T, Lerenthal Y, Moyal L, Andegeko Y, Mittelman L, Shiloh Y
The EMBO journal 2003 Oct 15;22(20):5612-21
The EMBO journal 2003 Oct 15;22(20):5612-21
Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage.
Lukas C, Falck J, Bartkova J, Bartek J, Lukas J
Nature cell biology 2003 Mar;5(3):255-60
Nature cell biology 2003 Mar;5(3):255-60
NFBD1/KIAA0170 is a chromatin-associated protein involved in DNA damage signaling pathways.
Xu X, Stern DF
The Journal of biological chemistry 2003 Mar 7;278(10):8795-803
The Journal of biological chemistry 2003 Mar 7;278(10):8795-803
Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks.
Celeste A, Fernandez-Capetillo O, Kruhlak MJ, Pilch DR, Staudt DW, Lee A, Bonner RF, Bonner WM, Nussenzweig A
Nature cell biology 2003 Jul;5(7):675-9
Nature cell biology 2003 Jul;5(7):675-9
Distinct functions of Nijmegen breakage syndrome in ataxia telangiectasia mutated-dependent responses to DNA damage.
Lee JH, Xu B, Lee CH, Ahn JY, Song MS, Lee H, Canman CE, Lee JS, Kastan MB, Lim DS
Molecular cancer research : MCR 2003 Jul;1(9):674-81
Molecular cancer research : MCR 2003 Jul;1(9):674-81
MDC1 is a mediator of the mammalian DNA damage checkpoint.
Stewart GS, Wang B, Bignell CR, Taylor AM, Elledge SJ
Nature 2003 Feb 27;421(6926):961-6
Nature 2003 Feb 27;421(6926):961-6
Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex.
Stracker TH, Carson CT, Weitzman MD
Nature 2002 Jul 18;418(6895):348-52
Nature 2002 Jul 18;418(6895):348-52
UV-induced replication arrest in the xeroderma pigmentosum variant leads to DNA double-strand breaks, gamma -H2AX formation, and Mre11 relocalization.
Limoli CL, Giedzinski E, Bonner WM, Cleaver JE
Proceedings of the National Academy of Sciences of the United States of America 2002 Jan 8;99(1):233-8
Proceedings of the National Academy of Sciences of the United States of America 2002 Jan 8;99(1):233-8
Interaction of FANCD2 and NBS1 in the DNA damage response.
Nakanishi K, Taniguchi T, Ranganathan V, New HV, Moreau LA, Stotsky M, Mathew CG, Kastan MB, Weaver DT, D'Andrea AD
Nature cell biology 2002 Dec;4(12):913-20
Nature cell biology 2002 Dec;4(12):913-20
Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome.
Harfst E, Cooper S, Neubauer S, Distel L, Grawunder U
Molecular immunology 2000 Oct;37(15):915-29
Molecular immunology 2000 Oct;37(15):915-29
Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome.
Harfst E, Cooper S, Neubauer S, Distel L, Grawunder U
Molecular immunology 2000 Oct;37(15):915-29
Molecular immunology 2000 Oct;37(15):915-29
ATM-dependent phosphorylation of nibrin in response to radiation exposure.
Gatei M, Young D, Cerosaletti KM, Desai-Mehta A, Spring K, Kozlov S, Lavin MF, Gatti RA, Concannon P, Khanna K
Nature genetics 2000 May;25(1):115-9
Nature genetics 2000 May;25(1):115-9
Nijmegen breakage syndrome disease protein and MRE11 at PML nuclear bodies and meiotic telomeres.
Lombard DB, Guarente L
Cancer research 2000 May 1;60(9):2331-4
Cancer research 2000 May 1;60(9):2331-4
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J
Genes & development 2000 Apr 15;14(8):927-39
Genes & development 2000 Apr 15;14(8):927-39
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mre11 Antibody (12D7) [NB100-473] - Analysis of human MRE11 expression by western blot in HeLa (1) and Raji (2) whole cell extract s using anti-MRE11 monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mre11 Antibody (12D7) [NB100-473] - Analysis. A. 30 ug Jurkat whole cell extract B. 30 ug Raji whole cell extract C. 30 ug NCI-H929 whole cell extract 7.5 % SDS-PAGE Mre11 antibody) dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mre11 Antibody (12D7) [NB100-473] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. A band appears at the theoretical molecular weight of 80 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mre11 Antibody (12D7) [NB100-473] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mre11 Antibody (12D7) [NB100-473] - Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of human Mre11-transfected 293T cells. 7.5% SDS-PAGE. Mre11 antibody [12D7] (NB100-473). HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Bands are prominent at the theoretical molecular weight of 80 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mre11 Antibody (12D7) [NB100-473] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (NB100-473). HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. A band appears at the theoretical molecular weight of 80 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Mre11 Antibody (12D7) [NB100-473] - Human primary cells stained with Mre11 antibody at a dilution of 1:60. cells were fixed in 1:1 Acetone/Methanol. Image from verified customer review.
