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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF2284 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Mre11 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Mre11 in Western blots.
- Reactivity
- Human
- Host
- Chicken/Avian
- Conjugate
- Unconjugated
- Antigen sequence
P49959- Isotype
- IgY
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references After double-strand break induction by UV-A, homologous recombination and nonhomologous end joining cooperate at the same DSB if both systems are available.
Rapp A, Greulich KO
Journal of cell science 2004 Oct 1;117(Pt 21):4935-45
Journal of cell science 2004 Oct 1;117(Pt 21):4935-45
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Mre11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Mre11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2284) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for Mre11 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
