MA5-34943

antibody from Invitrogen Antibodies

Targeting: C7

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: MA5-34943 - Provider product page
Provider: Invitrogen Antibodies
Product name: C7 Monoclonal Antibody (15D1)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Positive Control: HepG2 cell, K562 cell, HUVEC cell, HCT116 cell, SHG-44 cell.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 15D1
Vial size: 100 μL
Concentration: 2 mg/mL
Storage: -20°C, Avoid Freeze/Thaw Cycles, store in dark

C7 protein structure - MA5-34943 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of C7 in HCT116 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with C7 monoclonal antibody (Product # MA5-34943) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of C7 in HepG2 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with C7 monoclonal antibody (Product # MA5-34943) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of C7 in SHG-44 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with C7 monoclonal antibody (Product # MA5-34943) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of C7 in HCT116 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with C7 monoclonal antibody (Product # MA5-34943) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of C7 in HCT116 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with C7 monoclonal antibody (Product # MA5-34943) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of C7 in HepG2 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with C7 monoclonal antibody (Product # MA5-34943) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of C7 in SHG-44 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with C7 monoclonal antibody (Product # MA5-34943) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.