PA5-64583

antibody from Invitrogen Antibodies

Targeting: FGR c-fgr, p55c-fgr, SRC2

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-64583

Provider

Invitrogen Antibodies

Product name

Phospho-FGR (Tyr412) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Phospho-FGR (Tyr412) Polyclonal Antibody detects endogenous levels of FGR only when phosphorylated at Tyr412.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Bletinib ameliorates neutrophilic inflammation and lung injury by inhibiting Src family kinase phosphorylation and activity.

Kao TI, Chen PJ, Wang YH, Tseng HH, Chang SH, Wu TS, Yang SH, Lee YT, Hwang TL
British journal of pharmacology 2021 Oct;178(20):4069-4084

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Ionizing irradiation-induced Fgr in senescent cells mediates fibrosis.

Mukherjee A, Epperly MW, Shields D, Hou W, Fisher R, Hamade D, Wang H, Saiful Huq M, Bao R, Tabib T, Monier D, Watkins S, Calderon M, Greenberger JS
Cell death discovery 2021 Nov 12;7(1):349

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FGR in Mouse braintissue lysates using a Phospho-FGR (Tyr412) Polyclonal Antibody (Product # PA5-64583).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 4 Bletinib inhibits phosphorylation and enzymic activity of SKFs. (a-f) Phosphorylation of SFKs, namely, (a) Src, (b) Lyn, (c) Fgr, and (d) Hck, and downstream proteins, (e) Btk and (f) Vav, was determined through immunoblotting. (g-j) The ADP-Glo kinase assay kit was used to evaluate the enzymic activity. (g) Src, (h) Lyn, (i) Fgr, or (j) Hck (1.5 ng*ml -1 ) was incubated with DMSO, 1-10 muM bletinib, or 0.1-3 muM PP2, and then 125 muM ATP (substrate) was added to the reaction mixture for 60 min, which was followed by enzymic activity detection. Data are shown as box (interquartile range) and whiskers (min, max) plots, with medians ( n = 6). * P < 0.05, significantly different from the DMSO + fMLP group

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 In transwell culture, inhibition of tyrosine kinase Fgr by TL02-59 abrogates the induction of profibrotic genes in target cells. A Irradiated tdTOMp16+ non-senescent (tdTOM-) and senescent (tdTOM + ) cells were cultured on the top well of the transwell, and the target C57BL/6 stromal cells were cultured on the bottom well. Cells were treated with either vehicle or 10 nM Fgr inhibitor TL02-59 and after 10 days, target cells were harvested for RNA, and RT-qPCR was performed for TGF-beta and Collagen 3 genes. B Inhibition of Fgr phosphorylation by TL02-59 was confirmed by western blot from the vehicle and TL02-59-treated cell lysates of irradiated senescent (tdTOM + ) cells ( n = 3, * P =