Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-64583 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-FGR (Tyr412) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Phospho-FGR (Tyr412) Polyclonal Antibody detects endogenous levels of FGR only when phosphorylated at Tyr412.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Bletinib ameliorates neutrophilic inflammation and lung injury by inhibiting Src family kinase phosphorylation and activity.
Ionizing irradiation-induced Fgr in senescent cells mediates fibrosis.
Kao TI, Chen PJ, Wang YH, Tseng HH, Chang SH, Wu TS, Yang SH, Lee YT, Hwang TL
British journal of pharmacology 2021 Oct;178(20):4069-4084
British journal of pharmacology 2021 Oct;178(20):4069-4084
Ionizing irradiation-induced Fgr in senescent cells mediates fibrosis.
Mukherjee A, Epperly MW, Shields D, Hou W, Fisher R, Hamade D, Wang H, Saiful Huq M, Bao R, Tabib T, Monier D, Watkins S, Calderon M, Greenberger JS
Cell death discovery 2021 Nov 12;7(1):349
Cell death discovery 2021 Nov 12;7(1):349
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGR in Mouse braintissue lysates using a Phospho-FGR (Tyr412) Polyclonal Antibody (Product # PA5-64583).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 Bletinib inhibits phosphorylation and enzymic activity of SKFs. (a-f) Phosphorylation of SFKs, namely, (a) Src, (b) Lyn, (c) Fgr, and (d) Hck, and downstream proteins, (e) Btk and (f) Vav, was determined through immunoblotting. (g-j) The ADP-Glo kinase assay kit was used to evaluate the enzymic activity. (g) Src, (h) Lyn, (i) Fgr, or (j) Hck (1.5 ng*ml -1 ) was incubated with DMSO, 1-10 muM bletinib, or 0.1-3 muM PP2, and then 125 muM ATP (substrate) was added to the reaction mixture for 60 min, which was followed by enzymic activity detection. Data are shown as box (interquartile range) and whiskers (min, max) plots, with medians ( n = 6). * P < 0.05, significantly different from the DMSO + fMLP group
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 In transwell culture, inhibition of tyrosine kinase Fgr by TL02-59 abrogates the induction of profibrotic genes in target cells. A Irradiated tdTOMp16+ non-senescent (tdTOM-) and senescent (tdTOM + ) cells were cultured on the top well of the transwell, and the target C57BL/6 stromal cells were cultured on the bottom well. Cells were treated with either vehicle or 10 nM Fgr inhibitor TL02-59 and after 10 days, target cells were harvested for RNA, and RT-qPCR was performed for TGF-beta and Collagen 3 genes. B Inhibition of Fgr phosphorylation by TL02-59 was confirmed by western blot from the vehicle and TL02-59-treated cell lysates of irradiated senescent (tdTOM + ) cells ( n = 3, * P =