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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunocytochemistry [1]
- Other assay [3]
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- Product number
- PA1-738 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNAP23 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-738 detects synaptosomal-associated protein 23 (SNAP-23) from human, mouse and rat samples. PA1-738 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~23 kDa protein representing SNAP-23 from rat/mouse brain protein extract and HeLa cell lysates. PA1-738 immunizing peptide corresponds to amino acid residues 193-210 from mouse SNAP-23 protein. PA1-738 immunizing peptide (Cat. # PEP-147) is available for use in neutralization and control experiments.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis.
Developmental changes in the milk fat globule membrane proteome during the transition from colostrum to milk.
Effects of endurance exercise training on insulin signaling in human skeletal muscle: interactions at the level of phosphatidylinositol 3-kinase, Akt, and AS160.
Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis.
Wiseman DA, Kalwat MA, Thurmond DC
The Journal of biological chemistry 2011 May 6;286(18):16344-54
The Journal of biological chemistry 2011 May 6;286(18):16344-54
Developmental changes in the milk fat globule membrane proteome during the transition from colostrum to milk.
Reinhardt TA, Lippolis JD
Journal of dairy science 2008 Jun;91(6):2307-18
Journal of dairy science 2008 Jun;91(6):2307-18
Effects of endurance exercise training on insulin signaling in human skeletal muscle: interactions at the level of phosphatidylinositol 3-kinase, Akt, and AS160.
Frøsig C, Rose AJ, Treebak JT, Kiens B, Richter EA, Wojtaszewski JF
Diabetes 2007 Aug;56(8):2093-102
Diabetes 2007 Aug;56(8):2093-102
Identification of SNAREs involved in synaptotagmin VII-regulated lysosomal exocytosis.
Rao SK, Huynh C, Proux-Gillardeaux V, Galli T, Andrews NW
The Journal of biological chemistry 2004 May 7;279(19):20471-9
The Journal of biological chemistry 2004 May 7;279(19):20471-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SNAP-23 was performed using 90% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SNAP23 Rabbit Polyclonal Antibody (Product # PA1-738) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
