PA5-28936

antibody from Invitrogen Antibodies

Targeting: SNAP23 HsT17016, SNAP23A, SNAP23B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28936

Provider

Invitrogen Antibodies

Product name

SNAP23 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain, rat brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SNAP23 was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SNAP23 Polyclonal Antibody (Product # PA5-28936) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SNAP23 Polyclonal Antibody (Product # PA5-28936). Sample (30 µg of whole cell lysate). A: H1299. 12% SDS PAGE. SNAP23 Polyclonal Antibody (Product # PA5-28936) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SNAP23 in methanol-fixed HeLa cells using a SNAP23 polyclonal antibody (Product # PA5-28936) at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SNAP23 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP23 Polyclonal Antibody (Product # PA5-28936) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker. Blue: Fluoroshield with DAPI.