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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-31028 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNAP23 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, Raji, mouse brain, rat brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAP23 was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SNAP23 Polyclonal Antibody (Product # PA5-31028) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SNAP23 Polyclonal Antibody (Product # PA5-31028). Sample (30 µg of whole cell lysate). Lane A: A431. 12% SDS PAGE. SNAP23 Polyclonal Antibody (Product # PA5-31028) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SNAP23 in methanol-fixed HeLa cells using a SNAP23 polyclonal antibody (Product # PA5-31028) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SNAP23 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP23 Polyclonal Antibody (Product # PA5-31028) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using SNAP23 (Product # PA5-31028) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
