MA5-17150

antibody from Invitrogen Antibodies

Targeting: PIK3R1 GRB1, p85, p85-ALPHA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17150

Provider

Invitrogen Antibodies

Product name

PI3K p85 alpha Monoclonal Antibody (6G10)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17150 targets PIK3R1 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples. The MA5-17150 immunogen is purified recombinant fragment of human PIK3R1 (amino acids: 159-388) expressed in E. Coli. MA5-17150 detects PIK3R1 which has a predicted molecular weight of approximately 83.6kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

6G10

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIK3R1 using a PIK3R1 monoclonal antibody (Product # MA5-17150) against a human PIK3R1 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIK3R1 using a PIK3R1 monoclonal antibody (Product # MA5-17150) against a human PIK3R1 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIK3R1 using a PIK3R1 monoclonal antibody (Product # MA5-17150) against a human PIK3R1 recombinant protein.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using PIK3R1 monoclonal antibody (Product # MA5-17150) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 cells using PIK3R1 monoclonal antibody (Product # MA5-17150) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PIK3R1 monoclonal antibody (Product # MA5-17150) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIK3R1 monoclonal antibody (Product # MA5-17150) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of NIH3T3 cells using PIK3R1 monoclonal antibody (Product # MA5-17150) (green) and negative control (red).