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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- AF2998 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal PI 3-Kinase p85 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse and rat PI 3-Kinase p85 alpha in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis.
Han Y, Cui J, Tao J, Guo L, Guo P, Sun M, Kang J, Zhang X, Yan C, Li S
Experimental cell research 2009 Nov 15;315(19):3301-11
Experimental cell research 2009 Nov 15;315(19):3301-11
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3-Kinase p85 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2998) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3-Kinase p85 alpha at approximately 85 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
