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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3220 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3220, RRID:AB_1578773
- Product name
- PIK3R1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIK3R1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Genetic down-regulation of phosphoinositide 3-kinase by bikunin correlates with suppression of invasion and metastasis in human ovarian cancer HRA cells.
Polarity and proliferation are controlled by distinct signaling pathways downstream of PI3-kinase in breast epithelial tumor cells.
Activation of phosphatidylinositol 3-kinase/Akt pathway by androgen through interaction of p85alpha, androgen receptor, and Src.
Kobayashi H, Suzuki M, Kanayama N, Terao T
The Journal of biological chemistry 2004 Feb 20;279(8):6371-9
The Journal of biological chemistry 2004 Feb 20;279(8):6371-9
Polarity and proliferation are controlled by distinct signaling pathways downstream of PI3-kinase in breast epithelial tumor cells.
Liu H, Radisky DC, Wang F, Bissell MJ
The Journal of cell biology 2004 Feb 16;164(4):603-12
The Journal of cell biology 2004 Feb 16;164(4):603-12
Activation of phosphatidylinositol 3-kinase/Akt pathway by androgen through interaction of p85alpha, androgen receptor, and Src.
Sun M, Yang L, Feldman RI, Sun XM, Bhalla KN, Jove R, Nicosia SV, Cheng JQ
The Journal of biological chemistry 2003 Oct 31;278(44):42992-3000
The Journal of biological chemistry 2003 Oct 31;278(44):42992-3000
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3R1 polyclonal antibody (Cat # PAB3220) in 293 cell line lysates transiently transfected with the PIK3R1 gene (3 ug/lane). PI3KR1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3R1 polyclonal antibody (Cat # PAB3220) in CEM and Hela cell line lysates (35 ug/lane). PIK3R1 (arrow) was detected using the purified polyclonal antibody (1 : 63 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1 polyclonal antibody (Cat # PAB3220) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
