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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Proximity ligation assay [2]
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Validation data
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- Product number
- H00005295-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005295-D01P, RRID:AB_1711079
- Product name
- PIK3R1 purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human PIK3R1 protein.
- Antigen sequence
MHNLQTLPPKPPKPTTVANNGMNNNMSLQDAEWYW
GDISREEVNEKLRDTADGTFLVRDASTKMHGDYTL
TLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELI
NHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKED
NIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQ
EIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIE
KFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRL
EEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQY
LMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDED
LPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRE
SSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPY
NLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYA
QQRR- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PIK3R1 expression in transfected 293T cell line (H00005295-T01) by PIK3R1 MaxPab polyclonal antibody.Lane 1: PIK3R1 transfected lysate(50.00 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between PIK3R1 and SHC1. Huh7 cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between PIK3R1 and HRAS. HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-HRAS mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
