Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 697102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#697102, RRID:AB_2687110
- Product name
- Purified anti-PIK3R1
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W16101A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysates (15µg protein) from Jurkat, HeLa (negative control) and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 17µg/mL purified anti-PIK3R1 (clone W16101A) antibody and competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for clone W16101A and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: Molecular Weight ladder.
- Conjugate
- Unconjugated