697102

antibody from BioLegend

Targeting: PIK3R1 GRB1, p85, p85-ALPHA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

697102

Provider

BioLegend

Proper citation

BioLegend Cat#697102, RRID:AB_2687110

Product name

Purified anti-PIK3R1

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16101A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15µg protein) from Jurkat, HeLa (negative control) and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 17µg/mL purified anti-PIK3R1 (clone W16101A) antibody and competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for clone W16101A and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: Molecular Weight ladder.

Conjugate

Unconjugated