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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab121772 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant PI 3 Kinase p85 alpha _Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human PIK3R1 Antibody, Recombinant, could be used for WB, ICC, IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein FunctionBinds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
- Reactivity
- Mouse, Rat, Hamster
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 601-619- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant PI 3 Kinase p85 alpha Antibody (Ab121772) - Western Blot All lanes: Recombinant PI 3 Kinase p85 alpha Antibody (Ab121772) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution Predicted band size: 85 kDa Observed band size: 84 kDa Exposure time: 2.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant PI 3 Kinase p85 alpha Antibody (Ab121772) - ICC/IF Immunocytochemistry analysis of PI 3 Kinase p85 alpha (green) in MCF7 cells using Recombinant PI 3 Kinase p85 alpha Antibody (Ab121772) and cells were counterstained with DAPI (blue).
