- PA5-29220 - Invitrogen Antibodies PIK3R1 antibody

Submitted references Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.

Alzahrani AM, Rajendran P, Veeraraghavan VP, Hanieh H
International journal of molecular sciences 2021 Mar 23;22(6)

Kaempferol Inhibits Zearalenone-Induced Oxidative Stress and Apoptosis via the PI3K/Akt-Mediated Nrf2 Signaling Pathway: In Vitro and In Vivo Studies.

Rajendran P, Ammar RB, Al-Saeedi FJ, Mohamed ME, ElNaggar MA, Al-Ramadan SY, Bekhet GM, Soliman AM
International journal of molecular sciences 2020 Dec 28;22(1)

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PI3 kinase p85 alpha using 30 µg of A) Jurkat (B) K562 and C) THP-1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PI3 kinase p85 alpha polyclonal antibody (Product # PA5-29220) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PI3 kinase p85 alpha using 30 µg of BCL-1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PI3 kinase p85 alpha polyclonal antibody (Product # PA5-29220) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PIK3R1 in Various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with PIK3R1 Polyclonal antibody (Product # PA5-29220) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PI3K p85 alpha was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PI3K p85 alpha Polyclonal Antibody (Product # PA5-29220) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PI3K p85 alpha was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PI3K p85 alpha Polyclonal Antibody (Product # PA5-29220) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PI3K p85 alpha was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PI3K p85 alpha Polyclonal Antibody (Product # PA5-29220) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PI3K p85 alpha Polyclonal Antibody (Product # PA5-29220). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3K p85 alpha Polyclonal Antibody (Product # PA5-29220) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of PI3K p85 alpha was performed in paraffin-embedded mouse duodenum tissue using PI3K p85 alpha Polyclonal Antibody (Product # PA5-29220) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PI3K p85 alpha Polyclonal Antibody immunoprecipitates PI3 kinase p85 alpha protein in IP experiments. IP samples: Jurkat whole cell extract. A. Control with 5 µg of preimmune Rabbit IgG. B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 µg PI3K p85 alpha Polyclonal Antibody (Product # PA5-29220). 10 % SDS-PAGE. The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3K p85 alpha Polyclonal Antibody (Product # PA5-29220) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 KFL triggers the PI3K/Akt pathway in ZEA-induced HepG2 cells. ( A ) Cells were treated with KFL (10, 25 and 50 uM) followed by ZEA (40 uM) for 24 h. After the treatment, whole-cell lysates were exposed to Western blotting with anti-pPI3K and anti-pAkt antibodies. Total PI3K and Akt levels were measured as loading controls. ( B ) Cells were pre-treated with a PI3K/Akt inhibitor (LY294002, 30 muM) for 2 h, followed by KFL (50 muM) and/or ZEA (40 muM) for 24 h. Western blot was performed to detect the pAkt, HO-1 and Nrf2 levels by anti-pAkt, anti-HO-1 and anti-Nrf2 abs. Data are represented as the mean +- SD of triplicate values ( n = 3), and * p < 0.05 represents noteworthy discrepancies compared with the control. # p < 0.05 represents significant variations compared with the ZEA alone and KFL with ZEA treatment groups.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 KRL enhances cell survival mechanism. H9c2 cells were cultured in serum-free media for 3 h, followed by treatment with KRL for 2 h before or after DOX treatment, respectively. ( A , B ) KRL activates the IGF1R-mediated survival pathway in H9c2 cells. The expression of p-IGF1R, PI3K, p-PI3K, AKT, and p-AKT was analyzed by Western blotting. beta-actin was used as the internal control. ( C ) Representative Western blots showing the changes in MAPK signaling proteins (pP38 and pJNK) in H9c2 cells. Data are represented as the mean +- SD of triplicate values ( n = 3) and * p < 0.05 represents significant variations compared with the control. # p < 0.05 represents significant variations as compared to DOX alone and KRL with DOX treatment groups.

PA5-29220

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