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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- PA5-109261 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fibulin 5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Human skin tissue, JAR, SiHa, SKOV-3, rat testis tissue, mouse ovary tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fibulin 5 in human skin tissue lysate. Samples were incubated with Fibulin 5 polyclonal antibody (Product # PA5-109261), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Fibulin 5 in SKOV-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Fibulin 5 polyclonal antibody (Product # PA5-109261), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Fibulin 5 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Fibulin 5 polyclonal antibody (Product # PA5-109261), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Fibulin 5 in JAR cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Fibulin 5 polyclonal antibody (Product # PA5-109261), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Fibulin 5 in paraffin-embedded rat testis tissue. Samples were incubated with Fibulin 5 polyclonal antibody (Product # PA5-109261), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Fibulin 5 in paraffin-embedded mouse ovary tissue. Samples were incubated with Fibulin 5 polyclonal antibody (Product # PA5-109261), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Fibulin 5 in SiHa cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Fibulin 5 polyclonal antibody (Product # PA5-109261) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
