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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [6]
- Other assay [1]
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Validation data
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- Product number
- 12188-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fibulin 5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.
- Concentration
- 0.37 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse lung tissue slide using 12188-1-AP ( Fibulin 5 antibody at dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse lung tissue slide using 12188-1-AP ( Fibulin 5 antibody at dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Fibulin 5 (IP:12188-1-AP, 3ug; Detection:12188-1-AP 1:300) with mouse heart tissue lysate 8000ug.
