66258-1-Ig

antibody from Proteintech Group

Targeting: PTK2 FADK, FAK, FAK1, PPP1R71

Provider product page for 66258-1-Ig

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Immunohistochemistry

Antibody data

Antibody Data
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Product number: 66258-1-Ig - Provider product page
Provider: Proteintech Group
Product name: FAK antibody
Antibody type: Monoclonal
Description: KD/KO validated FAK antibody (Cat. #66258-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 2C5B9
Vial size: 20ul, 150ul

PTK2 protein structure - 66258-1-Ig shown in red.

Submitted references The brain imaging feature-related gene NRP2 drives the malignant progression of glioblastoma through the FAK pathway: a Mendelian randomization study.

Li Y, Que J, Xia Y, Wang L, Zhang P, Cheng Z, Gao B
Translational oncology 2026 May;67:102732

Mechanically heterogeneous hydrogel with cell-programmed network restructuring promotes tissue regeneration by mechano-epigenetic modulation.

Ling Q, Li H, Zhao J, Guo W, Yu H, Li Z, Hu Y, Wei S, Fu Y, Zhang Y, Zhang K, Bian L
Nature communications 2026 Jan 30;17(1)

AKAP2 is required for assembly of cytoskeletal signaling complexes that promote growth and metastasis of triple-negative breast cancer.

Rosenthal KJ, Felix-Sanchez P, Forbush K, Rhoads N, Kang M, Vicente JJ, Smith FD, Wordeman L, Ong SE, Cheung KJ, Scott JD
The Journal of biological chemistry 2026 Apr;302(4):111329

OCIAD2 Stabilizes Integrin β1 Signaling Through SNX17-Mediated Endosomal Recycling to Lipid Rafts and Modulates Cisplatin Response in HNSCC.

Cui L, Si S, Ye M, Lin P, Zou M, Lin Y, Chen X, Guo B, Sun W, Zhao X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Apr;13(20):e15452

Gut microbial metabolite, sphingosine-1-phosphate (S1P), drives mesangial cell phenotypic transformation and accelerates progression of IgA nephropathy via CCL2-MET-FAK pathway.

Tang YY, Zhang DL, Huang LS, Hu P, Liu P, Wu JJ, Xie T, Sun WQ, Xu XD, Jin MP, He HD
Current research in microbial sciences 2025;9:100494

Leucine-rich repeat neuronal 1 as a prognostic indicator and functional modulator in breast cancer.

Wang J, Zhou X, Ping D, Lu M, Zhang Y, Song H, Zhao J, Li D
Frontiers in oncology 2025;15:1724785

Low hydrostatic pressure promotes functional homeostasis of nucleus pulposus cells through the TRPV4/CRT/FA complex axis.

Hu J, Zhu Y, Pang Z, Li X, Zhang H, Li X, Gao Y, Wang Y, Li P, Zhou Q
Frontiers in medicine 2025;12:1531907

NSun2-Mediated tsRNAs Alleviate Liver Fibrosis via FAK Dephosphorylation.

Li P, Ying S, Zou Y, Wang X, Zhang R, Huang C, Dai M, Xu K, Feng G, Li X, Jiang H, Li Z, Zhang Y, Li W, Zhou Q
Cell proliferation 2025 Oct;58(10):e70058

ITGA5 drives angiogenesis in diabetic retinopathy via TAK-1/NF-kB activation.

Kan F, Wang D, Li S, Gao Y, Wang J
Human cell 2025 May 23;38(4):105

Targeting the hydrophobic pockets of FAK/PYK2 FAT domain: a highly effective inhibitory strategy suppressing tumor growth and eliminating metastasis.

Christoforou M, Charalambous A, Sfakianakis D, Skourides PA
Cell communication and signaling : CCS 2025 May 19;23(1):231

FAK and p130Cas Modulate Stiffness-Mediated Early Transcription and Cellular Metabolism.

Tumenbayar BI, Pham K, Biber JC, Tutino VM, Brazzo JA 3rd, Yao P, Bae Y
Cytoskeleton (Hoboken, N.J.) 2025 Mar;82(3):197-215

N-cadherin-triggered myosin II inactivation provides tumor cells with a mechanical cell competition advantage and chemotherapy resistance.

Dai Z, Chen S, Shi J, Rui M, Xu Q
Developmental cell 2025 Jun 23;60(12):1784-1801.e6

Consolidation of cell-ECM adhesion through direct talin-mediated actin linkage is essential for mouse embryonic morphogenesis.

Deng W, Carr RL, Kaul RR, Pavlova-Deb M, Haage A, Roca-Cusachs P, Tanentzapf G
Communications biology 2025 Jun 21;8(1):948

FBP1 as a key regulator of focal adhesion kinase-mediated hepatic stellate cell activation: Multi-omics and experimental validation.

Wu HY, Han L, Ran T, Sun Y, Zhang QX, Huang T, Zou GL, Zhang Y, Zhou YM, Lin GY, Chen SJ, Wang JL, Pan C, Lu F, Pu HF, Zhao XK
World journal of gastroenterology 2025 Jul 28;31(28):107361

In situ osteogenic activation of mesenchymal stem cells by the blood clot biomimetic mechanical microenvironment.

He W, Ding F, Zhang L, Liu W
Nature communications 2025 Jan 30;16(1):1162

Photo-tunable hydrogels reveal cellular sensing of rapid rigidity changes through the accumulation of mechanical signaling molecules.

Yang J, Wang P, Zhang Y, Zhang M, Sun Q, Chen H, Dong L, Chu Z, Xue B, Hoff WD, Zhao C, Wang W, Wei Q, Cao Y
Cell stem cell 2025 Jan 2;32(1):121-136.e6

Tenvermectin B, a novel macrocyclic lactone antibiotic, suppresses glioblastoma progression by targeting RhoJ.

Nie C, Liang H, Zhou J, Liu H, Shang R, Yang H, Jiang W, Qi H, Wang J, Gao A
Cellular signalling 2025 Dec;136:112117

IFIT3 promotes lymph node metastasis by interacting with LASP1 to activate FAK-ERK signaling in esophageal squamous cell carcinoma.

Cui Q, Zhou X, Chen X, Zhao X, Zhang R, Wang J, Li X, Wang G, Li B, Li M, Chen J, Gao X, Shi Y, Li W, Hong P, Li D
Cell death & disease 2025 Dec 18;17(1):110

c-Rel drives pancreatic cancer metastasis through fibronectin-integrin signaling-induced isolation stress resistance and EMT.

Bakırdöğen D, Görgülü K, Xin J, Richter L, Alcalá S, Ruiz-Cañas L, Dai C, Frank KJ, Wu N, Diakopoulos KN, Ozturk H, Demircioğlu D, Peschke K, Ranjan R, Fusco F, Martinez-Useros J, Fernandez-Aceñero MJ, Chhabra NF, López-Gil JC, Ai J, Ruess DA, Kaya-Aksoy E, Schmidt F, Kohlmann L, Berninger A, Yang H, Schicktanz F, Steiger K, Demir IE, Schmid RM, Reichert M, Adli M, Lesina M, Sainz B Jr, Algül H
Molecular cancer 2025 Dec 15;25(1):16

Exosomal circZNF800 Derived from Glioma Stem-like Cells Regulates Glioblastoma Tumorigenicity via the PIEZO1/Akt Axis.

Zhang N, Wu P, Mu M, Niu C, Hu S
Molecular neurobiology 2024 Sep;61(9):6556-6571

CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2.

Wang D, Li Y, Chang W, Feng M, Yang Y, Zhu X, Liu Z, Fu Y
Cell death & disease 2024 Sep 27;15(9):693

OTUD6B promotes cholangiocarcinoma growth by regulating STAT3 phosphorylation through deubiquitination of PTK2.

Xing G, Chen H, Guo Z, Cui Y, Li Y, Shen J
Cell biology international 2024 Nov;48(11):1766-1778

Hyperglycemic environments directly compromise intestinal epithelial barrier function in an organoid model and hyaluronan (∼35 kDa) protects via a layilin dependent mechanism.

Jatana S, Abbadi A, West GA, Ponti AK, Braga-Neto MB, Smith JL, Marino-Melendez A, Willard B, Nagy LE, Motte C
Matrix biology : journal of the International Society for Matrix Biology 2024 Nov;133:116-133

Three-Dimensional Semiconductor Network as Regulators of Energy Metabolism Drives Angiogenesis in Bone Regeneration.

Fan Y, Sun J, Fan W, Zhong X, Yin Z, Su B, Yao J, Hong X, Zhai J, Wang Z, Chen H, Guo F, Wen X, Ning C, Chen L, Yu P
ACS nano 2024 Nov 26;18(47):32602-32616

Spatial organization of PI3K-PI(3,4,5)P(3)-AKT signaling by focal adhesions.

Wang J, An Z, Wu Z, Zhou W, Sun P, Wu P, Dang S, Xue R, Bai X, Du Y, Chen R, Wang W, Huang P, Lam SM, Ai Y, Liu S, Shui G, Zhang Z, Liu Z, Huang J, Fang X, He K
Molecular cell 2024 Nov 21;84(22):4401-4418.e9

Targeting DUSP26 to drive cardiac mitochondrial dynamics via FAK-ERK signaling in diabetic cardiomyopathy.

Liu C, Xu X, Sun G, Song C, Jiang S, Sun P, Tian J
Free radical biology & medicine 2024 Nov 20;225:856-870

A decrease in integrin α5β1/FAK is associated with increased apoptosis of aortic smooth muscle cells in acute type a aortic dissection.

Xue M, Xing L, Yang Y, Shao M, Liao F, Xu F, Chen Y, Wang S, Chen B, Yao C, Gu G, Tong C
BMC cardiovascular disorders 2024 Mar 26;24(1):180

Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma.

Shi Q, Xu J, Chen C, Hu X, Wang B, Zeng F, Ren T, Huang Y, Guo W, Tang X, Ji T
Cancer letters 2024 Jun 1;591:216902

Muscone restores anoikis sensitivity in TMZ-resistant glioblastoma cells by suppressing TOP2A via the EGFR/Integrin β1/FAK signaling pathway.

Zou Y, Xu L, Wang W, Zhu X, Lin J, Li H, Chen J, Xu W, Gao H, Wu X, Yin Z, Wang Q
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jul;129:155714

Immunohistochemistry identifies E-cadherin, N-cadherin and focal adhesion kinase (FAK) as predictors of stage I non-small cell lung carcinoma spread through the air spaces (STAS), and the combinations as prognostic factors.

Meng Y, Wang Y, Liu L, Wu R, Zhang Q, Chen Z, Yao Y, Li X, Gong Y, Li H, Wang Z, Liu H
Translational lung cancer research 2024 Jul 30;13(7):1450-1462

Caveolin 1 Regulates the Tight Junctions between Sertoli Cells and Promotes the Integrity of Blood-Testis Barrier in Yak via the FAK/ERK Signaling Pathway.

Yan Q, Li T, Zhang Y, Zhao X, Wang Q, Yuan L
Animals : an open access journal from MDPI 2024 Jan 5;14(2)

Simultaneous inhibition of FAK and ROS1 synergistically repressed triple-negative breast cancer by upregulating p53 signalling.

Tan X, Kong D, Tao Z, Cheng F, Zhang B, Wang Z, Mei Q, Chen C, Wu K
Biomarker research 2024 Jan 25;12(1):13

Arctigenin promotes mucosal healing in ulcerative colitis through facilitating focal adhesion assembly and colonic epithelial cell migration via targeting focal adhesion kinase.

Guo Y, Liu X, Tao Y, Zhu Y, Zhang J, Yu X, Guo P, Liu S, Wei Z, Dai Y, Xia Y
International immunopharmacology 2024 Feb 15;128:111552

ACE2 improves endothelial cell function and reduces acute lung injury by downregulating FAK expression.

He Y, Gang B, Zhang M, Bai Y, Wan Z, Pan J, Liu J, Liu G, Gu W
International immunopharmacology 2024 Feb 15;128:111535

CSE1L in enhancing the effect of defactinib on gastric cancer cells via the inhibition of FAK phosphorylation.

He X, Wang Y, Zhang Y, Sahu A, Almhanna K, Liu Y
Translational cancer research 2024 Dec 31;13(12):6905-6918

Matricellular Protein SMOC2 Potentiates BMP9-Induced Osteogenic Differentiation in Mesenchymal Stem Cells through the Enhancement of FAK/PI3K/AKT Signaling.

He WG, Deng YX, Ke KX, Cao XL, Liu SY, Yang YY, Luo HH, Yao XT, Gao X, Du Y, He BC, Chen L
Stem cells international 2023;2023:5915988

The Effect of Adipocyte-Secreted Factors in Activating Focal Adhesion Kinase-Mediated Cell Signaling Pathway towards Metastasis in Breast Cancer Cells.

Mubtasim N, Gollahon L
International journal of molecular sciences 2023 Nov 22;24(23)

Integrin β1/FAK/SRC signal pathway is involved in autism spectrum disorder in Tspan7 knockout rats.

Pang S, Luo Z, Dong W, Gao S, Chen W, Liu N, Zhang X, Gao X, Li J, Gao K, Shi X, Guan F, Zhang L, Zhang L
Life science alliance 2023 Mar;6(3)

Cas phosphorylation regulates focal adhesion assembly.

Kumar S, Stainer A, Dubrulle J, Simpkins C, Cooper JA
eLife 2023 Jul 25;12

Fibronectin promotes tumor angiogenesis and progression of non-small-cell lung cancer by elevating WISP3 expression via FAK/MAPK/ HIF-1α axis and activating wnt signaling pathway.

Zhou F, Sun J, Ye L, Jiang T, Li W, Su C, Ren S, Wu F, Zhou C, Gao G
Experimental hematology & oncology 2023 Jul 19;12(1):61

γ-Bungarotoxin impairs the vascular endothelial barrier function by inhibiting integrin α5.

Chen W, Yu H, Sun C, Dong M, Zhao N, Wang Y, Yu K, Zhang J, Xu N, Liu W
Toxicology letters 2023 Jul 1;383:177-191

Lipid-anchored proteasomes control membrane protein homeostasis.

Zhang R, Pan S, Zheng S, Liao Q, Jiang Z, Wang D, Li X, Hu A, Li X, Zhu Y, Shen X, Lei J, Zhong S, Zhang X, Huang L, Wang X, Huang L, Shen L, Song BL, Zhao JW, Wang Z, Yang B, Guo X
Science advances 2023 Dec;9(48):eadj4605

miRNA-150_R-1 mediates the HIF-1/ErbB signaling pathway to regulate the adhesion of endometrial epithelial cells in cows experiencing retained placenta.

Lv C, Li Z, Wang Q, Wang Y, Zhao X, Zhang Y
Frontiers in veterinary science 2022;9:1037880

Astragalus propinquus schischkin and Salvia miltiorrhiza bunge promote angiogenesis to treat myocardial ischemia via Ang-1/Tie-2/FAK pathway.

Zhang MX, Huang XY, Song Y, Xu WL, Li YL, Li C
Frontiers in pharmacology 2022;13:1103557

Src kinase mediates coelomocytes phagocytosis via interacting with focal adhesion kinase in Vibrio splendidus challenged Apostichopus japonicus.

Wan J, Zhao X, Liu J, Chen K, Li C
Fish & shellfish immunology 2022 May;124:411-420

LMBR1L regulates the proliferation and migration of endothelial cells through Norrin/β-catenin signaling.

Liu W, Jiang X, Li X, Sun K, Yang Y, Yang M, Li S, Zhu X
Journal of cell science 2022 Mar 15;135(6)

Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition.

Hadjisavva R, Anastasiou O, Ioannou PS, Zheltkova M, Skourides PA
Cell reports 2022 Jul 19;40(3):111091

TNFSF9 promotes metastasis of pancreatic cancer by regulating M2 polarization of macrophages through Src/FAK/p-Akt/IL-1β signaling.

Wu J, Wang Y, Yang Y, Liu F, Jiang Z, Jiang Z
International immunopharmacology 2022 Jan;102:108429

AVL9 promotes colorectal carcinoma cell migration via regulating EGFR expression.

Wu Q, Chen J, Zhou Z
Biological procedures online 2022 Jan 6;24(1):1

LMOD1, an oncogene associated with Lauren classification, regulates the metastasis of gastric cancer cells through the FAK-AKT/mTOR pathway.

Tan Y, Chen Q, Pan S, An W, Xu H, Xing Y, Zhang J
BMC cancer 2022 Apr 30;22(1):474

MT1-MMP-dependent ECM processing regulates laminB1 stability and mediates replication fork restart.

Thakur V, Tiburcio de Freitas J, Li Y, Zhang K, Savadelis A, Bedogni B
PloS one 2021;16(7):e0253062

CDC37L1 acts as a suppressor of migration and proliferation in gastric cancer by down-regulating CDK6.

Li L, Tao X, Li Y, Gao Y, Li Q
Journal of Cancer 2021;12(11):3145-3153

TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages.

Wu J, Wang Y, Yang Y, Liu F, Chen J, Jiang Z, Jiang Z
Aging 2021 Sep 13;13(17):21571-21586

Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.

Ma S, Zhou J, Huang T, Zhang Z, Xing Q, Zhou X, Zhang K, Yao M, Cheng T, Wang X, Wen X, Guan F
Acta biomaterialia 2021 Sep 1;131:185-197

Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src.

Liu H, Zhou Y, Qiu H, Zhuang R, Han Y, Liu X, Qiu X, Wang Z, Xu L, Tan R, Hong W, Wang T
Cell death & disease 2021 Mar 17;12(4):284

NOL4L, a novel nuclear protein, promotes cell proliferation and metastasis by enhancing the PI3K/AKT pathway in ovarian cancer.

Lin F, Zhou J, Li X, Wang X
Biochemical and biophysical research communications 2021 Jun 25;559:121-128

FAK displacement from focal adhesions: a promising strategy to target processes implicated in cancer progression and metastasis.

Antoniades I, Kyriakou M, Charalambous A, Kalalidou K, Christodoulou A, Christoforou M, Skourides PA
Cell communication and signaling : CCS 2021 Jan 7;19(1):3

MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer.

Ma RR, Zhang H, Chen HF, Zhang GH, Tian YR, Gao P
Oncogene 2021 Apr;40(14):2524-2538

DHPAC, a novel microtubule depolymerizing agent, suppresses angiogenesis and vasculogenic mimicry formation of human non-small cell lung cancer.

Gong FL, Wang L, Yu LG, Dang YF, Jiang XN, Zhao L, Guo XL
Journal of cellular biochemistry 2020 Dec;121(12):4756-4771

TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling.

Wu X, Li S, Wang K, Hua W, Li S, Song Y, Zhang Y, Yang S, Yang C
Inflammation 2019 Oct;42(5):1575-1584

Subcutaneous and Visceral Adipose-Derived Mesenchymal Stem Cells: Commonality and Diversity.

Ritter A, Friemel A, Roth S, Kreis NN, Hoock SC, Safdar BK, Fischer K, Möllmann C, Solbach C, Louwen F, Yuan J
Cells 2019 Oct 21;8(10)

Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer.

Wang G, Yang Q, Li M, Zhang Y, Cai Y, Liang X, Fu Y, Xiao Z, Zhou M, Xie Z, Huang H, Huang Y, Chen Y, He Q, Peng F, Chen Z
Biology open 2019 May 13;8(5)

RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression.

Yu S, Gautam N, Quan M, Gao Y
Molecular medicine reports 2019 Aug;20(2):1747-1753

Rab34 regulates adhesion, migration, and invasion of breast cancer cells.

Sun L, Xu X, Chen Y, Zhou Y, Tan R, Qiu H, Jin L, Zhang W, Fan R, Hong W, Wang T
Oncogene 2018 Jul;37(27):3698-3714

Downregulation of STRAP promotes tumor growth and metastasis in hepatocellular carcinoma via reducing PTEN level.

Wu Q, Yu S, Chen J, Li Y, Gao Y
IUBMB life 2018 Feb;70(2):120-128

AmotP130 regulates Rho GTPase and decreases breast cancer cell mobility.

Chen ZL, Yang J, Shen YW, Li ST, Wang X, Lv M, Wang BY, Li P, Zhao W, Qiu RY, Liu Y, Liu PJ, Yang J
Journal of cellular and molecular medicine 2018 Apr;22(4):2390-2403

Downregulation of Talin1 promotes hepatocellular carcinoma progression through activation of the ERK1/2 pathway.

Chen P, Lei L, Wang J, Zou X, Zhang D, Deng L, Wu D
Cancer science 2017 Jun;108(6):1157-1168

Vascular protective effects of aqueous extracts of Tribulus terrestris on hypertensive endothelial injury.

Jiang YH, Guo JH, Wu S, Yang CH
Chinese journal of natural medicines 2017 Aug;15(8):606-614

β-Arrestin1 and Signal-transducing Adaptor Molecule 1 (STAM1) Cooperate to Promote Focal Adhesion Kinase Autophosphorylation and Chemotaxis via the Chemokine Receptor CXCR4.

Alekhina O, Marchese A
The Journal of biological chemistry 2016 Dec 9;291(50):26083-26097

Tumor-suppressive functions of long-chain acyl-CoA synthetase 4 in gastric cancer.

Ye X, Zhang Y, Wang X, Li Y, Gao Y
IUBMB life 2016 Apr;68(4):320-7

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