Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 44-596G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-FAK (Ser910) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Rab40-Cullin5 complex regulates EPLIN and actin cytoskeleton dynamics during cell migration.
Regulation of Focal Adhesion Kinase through a Direct Interaction with an Endogenous Inhibitor.
A multi-omics approach identifies key hubs associated with cell type-specific responses of airway epithelial cells to staphylococcal alpha-toxin.
MAP3K8/TPL-2/COT is a potential predictive marker for MEK inhibitor treatment in high-grade serous ovarian carcinomas.
SHOC2 and CRAF mediate ERK1/2 reactivation in mutant NRAS-mediated resistance to RAF inhibitor.
Meenhard Herlyn.
PLX4032, a selective BRAF(V600E) kinase inhibitor, activates the ERK pathway and enhances cell migration and proliferation of BRAF melanoma cells.
FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST.
Analyzing FAK and Pyk2 in early integrin signaling events.
Linklater ES, Duncan ED, Han KJ, Kaupinis A, Valius M, Lyons TR, Prekeris R
The Journal of cell biology 2021 Jul 5;220(7)
The Journal of cell biology 2021 Jul 5;220(7)
Regulation of Focal Adhesion Kinase through a Direct Interaction with an Endogenous Inhibitor.
Zak TJ, Koshman YE, Samarel AM, Robia SL
Biochemistry 2017 Sep 5;56(35):4722-4731
Biochemistry 2017 Sep 5;56(35):4722-4731
A multi-omics approach identifies key hubs associated with cell type-specific responses of airway epithelial cells to staphylococcal alpha-toxin.
Richter E, Harms M, Ventz K, Gierok P, Chilukoti RK, Hildebrandt JP, Mostertz J, Hochgräfe F
PloS one 2015;10(3):e0122089
PloS one 2015;10(3):e0122089
MAP3K8/TPL-2/COT is a potential predictive marker for MEK inhibitor treatment in high-grade serous ovarian carcinomas.
Gruosso T, Garnier C, Abelanet S, Kieffer Y, Lemesre V, Bellanger D, Bieche I, Marangoni E, Sastre-Garau X, Mieulet V, Mechta-Grigoriou F
Nature communications 2015 Oct 12;6:8583
Nature communications 2015 Oct 12;6:8583
SHOC2 and CRAF mediate ERK1/2 reactivation in mutant NRAS-mediated resistance to RAF inhibitor.
Kaplan FM, Kugel CH 3rd, Dadpey N, Shao Y, Abel EV, Aplin AE
The Journal of biological chemistry 2012 Dec 7;287(50):41797-807
The Journal of biological chemistry 2012 Dec 7;287(50):41797-807
Meenhard Herlyn.
Halaban R
Pigment cell & melanoma research 2010 Apr;23(2):287
Pigment cell & melanoma research 2010 Apr;23(2):287
PLX4032, a selective BRAF(V600E) kinase inhibitor, activates the ERK pathway and enhances cell migration and proliferation of BRAF melanoma cells.
Halaban R, Zhang W, Bacchiocchi A, Cheng E, Parisi F, Ariyan S, Krauthammer M, McCusker JP, Kluger Y, Sznol M
Pigment cell & melanoma research 2010 Apr;23(2):190-200
Pigment cell & melanoma research 2010 Apr;23(2):190-200
FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST.
Zheng Y, Xia Y, Hawke D, Halle M, Tremblay ML, Gao X, Zhou XZ, Aldape K, Cobb MH, Xie K, He J, Lu Z
Molecular cell 2009 Jul 10;35(1):11-25
Molecular cell 2009 Jul 10;35(1):11-25
Analyzing FAK and Pyk2 in early integrin signaling events.
Bernard-Trifilo JA, Lim ST, Hou S, Schlaepfer DD, Ilic D
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Phospho-FAK in extracts of human epithelial carcinoma cells expressing FAK with (2-5) or without (1) 100 ng/mL Taxol for 18-24 hours in serum-free media to arrest cells in mitosis. Samples were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to a PVDF membrane. Results indicate specificity for Phospho-FAK Ser910 (Product # 44-596G).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of FAK [pS910] was done on A549 cells treated with EGF (200ng/mL, 10 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with FAK [pS910] Rabbit Polyclonal Antibody (44596G, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 PLX4032 reduced cell adhesion and promoted migration in BRAF WT melanoma cells. (A) The histograms show the number of floating YUDOSO-BRAF WT melanoma cells at different time intervals (in hours) after the addition of PLX4032 as percent of control (2500 floating cells). (B) Changes in FAK activation in response to PLX4032, as detected by Western blotting with antibodies against phospho FAK (S910), FAK, phospho-ERK1/2, ERK1/2 and actin. (C) Low magnification photomicrographs of 10 days soft agar cultures showing YUDOSO-BRAF WT cells forming large colonies in control (DMSO), but single cells or small colonies in PLX4032. The histogram demonstrates the viability (MTS) of cells in 96-well soft agar plates measuring absorbance at 490 nm. (D) Cell migration in response to PLX4032. The histograms show the number of cells that migrated thorough the transwell pores by counting three microscopical fields after 8 h treatment (a), or by extracting the stained cells and measuring absorbance at 570 nm after 24 h incubation with the drug normalized to control, DMSO treated cells (b).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 MAP3K8 is upstream of MEK and involved in regulating cyclin D1 and FAK in ovarian cancer cells. ( a ) Representative western blots showing the phosphorylated form of MEK (P-MEK) and total MEK protein levels in SKOV3 and IGROV-1 OCCL either treated with MAP3K8 kinase inhibitor (KI) or with KI vehicle (DMSO), for 1 h before serum (FBS) stimulation for the indicated times. Actin is used as an internal control for protein loading. ( b ) Bar plots showing P-MEK/MEK ratio, as assessed by densitometry analysis of western blots (as shown in a ) and normalized to the DMSO t =0 h time point ( n =3). ( c ) Western blots showing MAP3K8, P-MEK and MEK protein levels in stable cell lines (shCtrl, shMAP3K8_1 and shMAP3K8_2) derived from SKOV3 and IGROV-1 OCCL and kept without serum (-FBS) or stimulated with serum (+FBS). Actin is used as an internal control for protein loading. ( d ) Bar graphs showing P-MEK/MEK ratio assessed by densitometry analysis of western blots (as shown in c ) and normalized to shCtrl ( n 3). ( e ) Representative western blots showing P-p90RSK, p90RSK, cyclin D1, P-FAK and FAK protein levels upon serum (FBS) stimulation for the indicated times in SKOV3 and IGROV-1 OCCL pretreated either with MAP3K8 KI or with KI vehicle (DMSO). Actin is used as an internal control for protein loading. ( f ) Bar plots showing P-p90RSK/p90RSK ratio, cyclin D1 and P-FAK/FAK ratio, as assessed by densitometry analysis of western blots (as shown in e ) and expressed as fold chan
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure S2. FA maturation analysis. (A) Images of control and FLAG-Rab40b-4A cells stained with zyxin (green) and paxillin (red). Boxes mark regions that are shown as higher-resolution images. (B) Quantification of percentage of zyxin-positive FAs in control and FLAG-Rab40b-4A cells. n >= 15 cells per cell line were analyzed. Control, 76.2 +- 2.9 SEM; FLAG-Rab40b-4A, 89.7 +- 2.1 SEM. (C) WB images of cell lysates blotted with anti-FAK, anti-Y397 pFAK, or anti-S910 pFAK antibodies. Numbers shown are the average densitometry analysis derived from three independent experiments relative to tubulin and standardized to control levels. Control::FLAG-Rab40b, S910 P = 0.05; Control::FLAG-Rab40b-4A, S910 P < 0.005, Y397 P < 0.05.