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- Validations
- Western blot [6]
- Immunocytochemistry [1]
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- Product number
- GTX132140 - Provider product page
- Provider
- GeneTex
- Product name
- FAK (phospho Tyr576) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein by western blot analysis. Left: Untreated (-) and EGF-treated (+, 50 ng/ml for 30 min) 293T whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE. Right: Untreated (-) and EGF-treated (+, 200 ng/ml for 30 min) HepG2 whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE. The membranes were blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:3000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FAK (phospho Tyr576) protein stained by FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
