GTX129840

antibody from GeneTex

Targeting: PTK2 FADK, FAK, FAK1, PPP1R71

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX129840

Provider

GeneTex

Product name

FAK (phospho Tyr397) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

A novel miR-365-3p/EHF/keratin 16 axis promotes oral squamous cell carcinoma metastasis, cancer stemness and drug resistance via enhancing β5-integrin/c-met signaling pathway.

Huang WC, Jang TH, Tung SL, Yen TC, Chan SH, Wang LH
Journal of experimental & clinical cancer research : CR 2019 Feb 19;38(1):89

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The Physicochemical Properties of Decellularized Extracellular Matrix-Coated 3D Printed Poly(ε-caprolactone) Nerve Conduits for Promoting Schwann Cells Proliferation and Differentiation.

Chen CC, Yu J, Ng HY, Lee AK, Chen CC, Chen YS, Shie MY
Materials (Basel, Switzerland) 2018 Sep 9;11(9)

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Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.

Terasaki M, Ogawa Y, Endo T, Maeda H, Hamada J, Osada K, Miyashita K, Mutoh M
Anticancer research 2018 Apr;38(4):2169-2179

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Fibronectin in cell adhesion and migration via N-glycosylation.

Hsiao CT, Cheng HW, Huang CM, Li HR, Ou MH, Huang JR, Khoo KH, Yu HW, Chen YQ, Wang YK, Chiou A, Kuo JC
Oncotarget 2017 Sep 19;8(41):70653-70668

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Induction of Anoikis in Human Colorectal Cancer Cells by Fucoxanthinol.

Terasaki M, Maeda H, Miyashita K, Mutoh M
Nutrition and cancer 2017 Oct;69(7):1043-1052

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Fucoidan inhibition of lung cancer in vivo and in vitro : role of the Smurf2-dependent ubiquitin proteasome pathway in TGFβ receptor degradation.

Hsu HY, Lin TY, Wu YC, Tsao SM, Hwang PA, Shih YW, Hsu J
Oncotarget 2014 Sep 15;5(17):7870-85

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) HUVEC whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (GTX129840) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (GTX129840) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (GTX129840) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (GTX129840) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (GTX129840) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (GTX129840) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.