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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP2-68146 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal FAK Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: FAK [p Tyr861] Antibody [NBP2-68146] - Extracts of 3T3-L1 cells unstimulated or stimulated with 50 ng/ml LIF for 15 minutes (2-4) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4C, then incubated with JAK2 (pYpY1007/1008) antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the phosphopeptide immunogen (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F (ab')2 anti-rabbit IgG HRP-conjugate and signals were detected using the Pierce SuperSignal method. The data show that only the phosphopeptide corresponding to JAK2 (pYpY1007/1008) blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the up-regulation of JAK2 (pYpY1007/1008) with LIF treatment in this cell system.
