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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F54458 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Focal Adhesion Kinase Antibody / FAK1 / PTK2
- Antibody type
- Polyclonal
- Description
- This highly specific Focal Adhesion Kinase antibody is suitable for use in Immunofluorescence/Flow cytometry/Immunohistochemistry/Western blot applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Focal Adhesion Kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human T47-D cell lysate with Focal Adhesion Kinase antibody. Predicted molecular weight ~119 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Focal Adhesion Kinase antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human HeLa cells with Focal Adhesion Kinase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human brain tissue with Focal Adhesion Kinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human K562 cells with Focal Adhesion Kinase antibody; Blue=isotype control, Green= Focal Adhesion Kinase antibody.
