- PA5-17084 - Invitrogen Antibodies PTK2 antibody

Submitted references Subendothelial stiffness alters endothelial cell traction force generation while exerting a minimal effect on the transcriptome.

Bastounis EE, Yeh YT, Theriot JA
Scientific reports 2019 Dec 3;9(1):18209

Distinct PKA regulatory subunits mediate PGE(2) inhibition of TGFβ-1-stimulated collagen I translation and myofibroblast differentiation.

Wettlaufer SH, Penke LR, Okunishi K, Peters-Golden M
American journal of physiology. Lung cellular and molecular physiology 2017 Oct 1;313(4):L722-L731

Focal adhesion kinase as a mechanotransducer during rapid brain growth of the chick embryo.

Desmond ME, Knepper JE, DiBenedetto AJ, Malaugh E, Callejo S, Carretero R, Alonso MI, Gato A
The International journal of developmental biology 2014;58(1):35-43

Green tea epigallocatechin gallate exhibits anticancer effect in human pancreatic carcinoma cells via the inhibition of both focal adhesion kinase and insulin-like growth factor-I receptor.

Vu HA, Beppu Y, Chi HT, Sasaki K, Yamamoto H, Xinh PT, Tanii T, Hara Y, Watanabe T, Sato Y, Ohdomari I
Journal of biomedicine & biotechnology 2010;2010:290516

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-FAK pTyr397 in extracts from NIH/3T3 cells, untransfected or transfected with activated Src, using Phospho-FAK pTyr397 polyclonal antibody (Product # PA5-17084) (upper) or a PTK2 polyclonal antibody (lower).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Post-transcriptional changes on ECs in monolayers grown on soft versus stiff hydrogels. ( a ) Western blots from whole HUVEC or HMEC-1 lysates of cells previously residing on soft gels (3 kPa) or stiff gels (70 kPa). Representative cropped blots are displayed and full-length blots can be found in the supplementary material (Supplementary Fig. S2 ). Each row shows a different protein whose expression, phosphorylation or conformation was probed, namely: active form of integrin beta1, total integrin beta1, p397FAK, total FAK, p-Vav2, total Vav2, p-p70S6K, total p70S6K, p-ERK, total ERK and GAPDH (used as loading control). Experiments were performed N = 3 times. ( b ) Bar plots show relative expression of the proteins probed in panel a for HUVEC cells residing on soft (blue) or stiff (red) gels. All measurements were normalized to GAPDH expression for each condition, and expressed as fold-change relative to the median expression level on soft substrates. One or two asterisks denote statistically significant differences between the medians of two distributions (

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4. PKA subunit RIIbeta agonist inhibits TGFbeta-1-stimulated phosphorylation of FAK and relative expression of SRF. A and B : reduced level of phosphorylation of FAK by PKARIIbeta agonist (Sp-5,6-DCI-cBIMPs) ( B ) but not PKARIbeta agonist (2-Cl-8-MA-cAMP) ( A ). After 24 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/ml), and cells were harvested 24 h thereafter for Western blot analysis. Blots are from one representative experiment, and graph shows mean and SE values from densitometric analysis of n = 4 independent experiments for p-FAK. *Significant difference from TGFbeta-1 alone, P < 0.001. C and D : inhibition of SRF mRNA ( C ) and protein ( D ) expression by PKARIIbeta agonist. After 48 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/ml), and cells were harvested 24 h thereafter for mRNA analysis by qPCR and protein analysis by Western blot. The graph shows mean and SE values from relative expression of n = 4 independent experiments. Densitometry of SRF protein blots was normalized to GAPDH. Relative expression of SRF was normalized to GAPDH. E : effect of R isoform-specific knockdown on PGE 2 inhibition of FAK protein phosphorylation by TGFbeta-1 After incubation for 4 days with R isoform-specific siRNA, HLFs were treated for 30 min with or without PGE 2 (500 nM) before addit

PA5-17084

Antibody data