MA5-15643

antibody from Invitrogen Antibodies

Targeting: PTK2 FADK, FAK, FAK1, PPP1R71

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15643

Provider

Invitrogen Antibodies

Product name

FAK Monoclonal Antibody (10H7A6)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15643 targets FAK in WB applications and shows reactivity with Human samples.

Antibody clone number

10H7A6

Concentration

Conc. Not Determined

References

Submitted references

Distinct PKA regulatory subunits mediate PGE(2) inhibition of TGFβ-1-stimulated collagen I translation and myofibroblast differentiation.

Wettlaufer SH, Penke LR, Okunishi K, Peters-Golden M
American journal of physiology. Lung cellular and molecular physiology 2017 Oct 1;313(4):L722-L731

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FAK using FAK monoclonal antibody (Product # MA5-15643) in FAK (AA: 354-533) human IgG Fc transfected HEK293 cell lysate.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4. PKA subunit RIIbeta agonist inhibits TGFbeta-1-stimulated phosphorylation of FAK and relative expression of SRF. A and B : reduced level of phosphorylation of FAK by PKARIIbeta agonist (Sp-5,6-DCI-cBIMPs) ( B ) but not PKARIbeta agonist (2-Cl-8-MA-cAMP) ( A ). After 24 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/ml), and cells were harvested 24 h thereafter for Western blot analysis. Blots are from one representative experiment, and graph shows mean and SE values from densitometric analysis of n = 4 independent experiments for p-FAK. *Significant difference from TGFbeta-1 alone, P < 0.001. C and D : inhibition of SRF mRNA ( C ) and protein ( D ) expression by PKARIIbeta agonist. After 48 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/ml), and cells were harvested 24 h thereafter for mRNA analysis by qPCR and protein analysis by Western blot. The graph shows mean and SE values from relative expression of n = 4 independent experiments. Densitometry of SRF protein blots was normalized to GAPDH. Relative expression of SRF was normalized to GAPDH. E : effect of R isoform-specific knockdown on PGE 2 inhibition of FAK protein phosphorylation by TGFbeta-1 After incubation for 4 days with R isoform-specific siRNA, HLFs were treated for 30 min with or without PGE 2 (500 nM) before addit