21402-1-AP

antibody from Proteintech Group

Targeting: UHRF1 FLJ21925, ICBP90, Np95, RNF106, TDRD22

Provider product page for 21402-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [21]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 21402-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21402-1-AP, RRID:AB_10860902
Product name: UHRF1 antibody
Antibody type: Polyclonal
Description: KD/KO validated UHRF1 antibody (Cat. #21402-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

UHRF1 protein structure - 21402-1-AP shown in red.

Submitted references YTHDF1 promotes gallbladder cancer progression via post-transcriptional regulation of the m6A/UHRF1 axis.

Chen J, Bai X, Zhang W, Yan Z, Liu Y, Zhou S, Wu X, He X, Yang A
Journal of cellular and molecular medicine 2024 May;28(9):e18328

Comprehensive analysis reveals the prognostic and immunogenic characteristics of DNA methylation regulators in lung adenocarcinoma.

Huang J, Huang C, Huang C, Xiang Z, Ni Y, Zeng J, Cai S
Respiratory research 2024 Feb 5;25(1):74

Upregulation of UHRF1 Promotes PINK1-mediated Mitophagy to Alleviates Ferroptosis in Diabetic Nephropathy.

Ji H, Zhao Y, Ma X, Wu L, Guo F, Huang F, Song Y, Wang J, Qin G
Inflammation 2024 Apr;47(2):718-732

Single-cell multi-omics sequencing of human spermatogenesis reveals a DNA demethylation event associated with male meiotic recombination.

Huang Y, Li L, An G, Yang X, Cui M, Song X, Lin J, Zhang X, Yao Z, Wan C, Zhou C, Zhao J, Song K, Ren S, Xia X, Fu X, Lan Y, Hu X, Wang W, Wang M, Zheng Y, Miao K, Bai X, Hutchins AP, Chang G, Gao S, Zhao XY
Nature cell biology 2023 Oct;25(10):1520-1534

Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy.

Kang Z, Fu P, Ma H, Li T, Lu K, Liu J, Ginjala V, Romanienko P, Feng Z, Guan M, Ganesan S, Xia B
bioRxiv : the preprint server for biology 2023 Oct 4;

PLK1 maintains DNA methylation and cell viability by regulating phosphorylation-dependent UHRF1 protein stability.

Peng Y, Liu Y, Zheng R, Ye Y, Fu Y, Yin L, Gao Y, Fu Y, Qi X, Deng T, Zhang S, Li X
Cell death discovery 2023 Oct 3;9(1):367

Diosgenin inhibits prostate cancer progression by inducing UHRF1 protein degradation.

Peng Y, Tang R, Ding L, Zheng R, Liu Y, Yin L, Fu Y, Deng T, Li X
European journal of pharmacology 2023 Mar 5;942:175522

Methionine restriction promotes cGAS activation and chromatin untethering through demethylation to enhance antitumor immunity.

Fang L, Hao Y, Yu H, Gu X, Peng Q, Zhuo H, Li Y, Liu Z, Wang J, Chen Y, Zhang J, Tian H, Gao Y, Gao R, Teng H, Shan Z, Zhu J, Li Z, Liu Y, Zhang Y, Yu F, Lin Z, Hao Y, Ge X, Yuan J, Hu HG, Ma Y, Qin HL, Wang P
Cancer cell 2023 Jun 12;41(6):1118-1133.e12

RNF106 aggravates esophageal squamous cell carcinoma progression through LATS2/YAP axis.

Sun Q, Lu H, Zhang W, Du Y, Liang Q, Zhang Y, Wu J, Zhong M
Archives of biochemistry and biophysics 2023 Jul 1;742:109640

Lenvatinib inhibited HCC cell migration and invasion through regulating the transcription and ubiquitination of UHRF1 and DNMT1.

Fang T, Jiao Z, You Y, Cao J, Wang C, Liu J, Zhao W
Biochemical pharmacology 2023 Apr;210:115489

UHRF1 interacts with snRNAs and regulates alternative splicing in mouse spermatogonial stem cells.

Zhou S, Dong J, Xiong M, Gan S, Wen Y, Zhang J, Wang X, Yuan S, Gui Y
Stem cell reports 2022 Aug 9;17(8):1859-1873

Simultaneous silencing Aurora-A and UHRF1 inhibits colorectal cancer cell growth through regulating expression of DNMT1 and STAT1.

Han J, Chen X, Xu J, Chu L, Li R, Sun N, Jiang Z, Liu H, Ge X, Zheng J, Yang J, Ikezoe T
International journal of medical sciences 2021;18(15):3437-3451

Silencing UHRF1 Enhances Radiosensitivity of Esophageal Squamous Cell Carcinoma by Inhibiting the PI3K/Akt/mTOR Signaling Pathway.

Hui B, Pan S, Che S, Sun Y, Yan Y, Guo J, Gong T, Ren J, Zhang X
Cancer management and research 2021;13:4841-4852

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Morani F, Bisceglia L, Rosini G, Mutti L, Melaiu O, Landi S, Gemignani F
International journal of molecular sciences 2021 Mar 8;22(5)

USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.

Li J, Wang R, Jin J, Han M, Chen Z, Gao Y, Hu X, Zhu H, Gao H, Lu K, Shao Y, Lyu C, Lai W, Li P, Hu G, Li J, Li D, Wang H, Wu Q, Wong J
Cell discovery 2020;6:58

A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.

Zhang Q, Cao C, Gong W, Bao K, Wang Q, Wang Y, Bi L, Ma S, Zhao J, Liu L, Tian S, Zhang K, Yang J, Yao Z, Song N, Shi L
Theranostics 2020;10(23):10769-10790

Deficiency of pseudogene UPAT leads to hepatocellular carcinoma progression and forms a positive feedback loop with ZEB1.

Xiang L, Huang X, Wang S, Ou H, Chen Z, Hu Z, Huang Y, Li X, Yuan Y, Yang D
Cancer science 2020 Nov;111(11):4102-4117

UHRF1 Promotes Proliferation of Human Adipose-Derived Stem Cells and Suppresses Adipogenesis via Inhibiting Peroxisome Proliferator-Activated Receptor γ.

Chen K, Guo Z, Luo Y, Yuan J, Mo Z
BioMed research international 2019;2019:9456847

SET8 prevents excessive DNA methylation by methylation-mediated degradation of UHRF1 and DNMT1.

Zhang H, Gao Q, Tan S, You J, Lyu C, Zhang Y, Han M, Chen Z, Li J, Wang H, Liao L, Qin J, Li J, Wong J
Nucleic acids research 2019 Sep 26;47(17):9053-9068

Activated MEK/ERK Pathway Drives Widespread and Coordinated Overexpression of UHRF1 and DNMT1 in Cancer cells.

Li J, Wang R, Hu X, Gao Y, Wang Z, Li J, Wong J
Scientific reports 2019 Jan 29;9(1):907

Quantitative proteomic analysis of the metastasis-inhibitory mechanism of miR-193a-3p in non-small cell lung cancer.

Deng W, Yan M, Yu T, Ge H, Lin H, Li J, Liu Y, Geng Q, Zhu M, Liu L, He X, Yao M
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2015;35(5):1677-88

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP(UHRF1 antibody) at dilution of 1:300
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using UHRF1 antibody 21402-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line