PA5-27969

antibody from Invitrogen Antibodies

Targeting: UHRF1 FLJ21925, ICBP90, Np95, RNF106, TDRD22

Provider product page for PA5-27969

Western blot

Immunocytochemistry

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [2]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [2]

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Product number: PA5-27969 - Provider product page
Provider: Invitrogen Antibodies
Product name: UHRF1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HeLa. Predicted reactivity: Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

UHRF1 protein structure - PA5-27969 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 LASP-1 associates with epigenetic proteins UHRF1, DNMT1, G9a and Snail1 A) LASP-1 co-immunoprecipitates with UHRF1 and DNMT1. 100 mug of the nuclear extracts from MDA-Bone-Un cells stimulated with vehicle (0 min) or 500 ng /ml of CXCL12 for 15 min were incubated with control mouse monoclonal IgG (Mock) or with mouse anti-LASP-1 antibody. LASP1-bound proteins were eluted and analyzed for endogenous LASP-1 and associated proteins UHRF1 and DNMT1. Bottom panel: 15 mug of total cell lysates from MDA-Bone-Un cells that were stimulated with vehicle (0 min) or CXCL12 for 15 min. LASP-1 lysate blot was obtained after stripping and re-probing for LASP-1 after the detection of Snail1 first. The experiment was repeated twice and the representative blot was quantified and shown. NS - Non-silenced control; KD - LASP-1 knock down. The band intensities of proteins were quantified by using Image J analysis and normalized to their respective non-stimulated control (bands in -CXCL12 lane). The fold change was given above the bands to track the level of the immunoprecipitated proteins with respect to their protein levels in the lysates. B) LASP-1 co-immunoprecipitates with G9a and Snail1. 100 mug of the nuclear extracts from MDA-Bone-Un cells stimulated with vehicle (0 min and Mock) or 500 ng /ml of CXCL12 for 15 min were incubated with control mouse monoclonal IgG (Mock) or with mouse anti-LASP-1 antibody. Bound proteins were eluted and analyzed for endogenous LASP-1 and associated G9a

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6. The expression and clinical significance of miR-9 to 1 and UHRF1 in NSCLC. (A) IHC assay to measure UHRF1 level in 56 NSCLC tissues and 36 adjacently normal lung tissues. (B) The correlation between UHRF1 and miR-9 to 1 level in 56 NSCLC tissues. (C) Kaplan-Meier survival analysis was used to evaluate the prognostic value of UHRF1 expression for OS of 56 NSCLC tissues. (D) Kaplan-Meier survival analysis to evaluate the prognostic value of UHRF1 together with miR-9 to 1 expression in NSCLC for OS. Abbreviations: NSCLC, nonsmall cell lung cancer; miR-9 to 1, microRNA-9 to 1; OS, overall survival; UHRF1, ubiquitin-like containing PHD and RING finger domains 1.