MA5-14839

antibody from Invitrogen Antibodies

Targeting: PTPN6 HCP, HCPH, PTP-1C, SHP-1, SHP1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14839

Provider

Invitrogen Antibodies

Product name

SHP-1 Monoclonal Antibody (R.488.4)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

R.488.4

Vial size

100 µL

Concentration

92 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-SHP-1 Monoclonal Antibody (R.488.4)(Product # MA5-14839) and a 63 kDa band corresponding to SHP-1 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), THP-1 (Lane 2), HEL 92.1.7 (Lane 3), K-562 (Lane 4), U-2 OS (Lane 5), Mouse Spleen (Lane 6), Rat Spleen (Lane 7), Mouse Skeletal Muscle (Lane 8), Rat Skeletal Muscle (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).