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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX102864 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102864, RRID:AB_1951541
- Product name
- SHP1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Schistosoma mansoni Soluble Egg Antigens Induce Expression of the Negative Regulators SOCS1 and SHP1 in Human Dendritic Cells via Interaction with the Mannose Receptor.
Klaver EJ, Kuijk LM, Lindhorst TK, Cummings RD, van Die I
PloS one 2015;10(4):e0124089
PloS one 2015;10(4):e0124089
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:HeLaS3B:Hep G2(GTX27900)7.5% SDS PAGEGTX102864 diluted at 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A:NIH-3T37.5% SDS PAGE GTX102864 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SHP1 antibody detects SHP1 protein by western blot analysis.A. 50 ?g rat liver extract7.5% SDS-PAGESHP1 antibody (GTX102864) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SHP1 antibody immunoprecipitates SHP1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 £gg whole cell lysate/extract of SHP1 protein expressing HeLa cells B : Control with 3 £gg of pre-immune rabbit IgG C : Immunoprecipitation of SHP1 by 3 £gg of SHP1 antibody (GTX102864) 7.5% SDS-PAGE The immunoprecipitated SHP1 protein was detected by SHP1 antibody (GTX102864) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using SHP1(GTX102864) antibody at 1:100 dilution.
