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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36682 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SHP-1 (Tyr536) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 68 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SHP-1 (Tyr536) in Lane 1: MCF-7 whole cell lysate (40 µg), Lane 2: H1792 whole cell lysate (40 µg), Lane 3: A549 whole cell lysate (40 µg), Lane 4: 3T3-L1 whole cell lysate (40 µg), Lane 5: H9C2 whole cell lysate (40 µg). Samples were incubated with Phospho-SHP-1 (Tyr536) polyclonal antibody (Product # PA5-36682) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SHP-1 pTyr536 using Phospho-SHP-1 pTyr536 polyclonal antibody (Product # PA5-36682) at a dilution of 1:500. Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: PC12 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-SHP-1 (Tyr536) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-SHP-1 (Tyr536) polyclonal antibody (Product # PA5-36682) at a dilution of 1:100.
