M00938

antibody from Boster Biological Technology

Targeting: PTPN6 HCP, HCPH, PTP-1C, SHP-1, SHP1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00938

Provider

Boster Biological Technology

Product name

Anti-SHP1 PTPN6 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for SHP 1/PTPN6 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human, Mouse, Rat SHP 1/PTPN6 information: Molecular Weight: 67561 MW; Subcellular Localization: Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1; Tissue Specificity: Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

CGH-16

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rat spleen, using SHP1 Antibody(M00938)PTPN6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PTPN6 Antibody (M00938)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image