- MA5-11669 - Invitrogen Antibodies PTPN6 antibody

Submitted references Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates.

Huang CF, Gottardi CJ, Mrksich M
Scientific reports 2022 Sep 5;12(1):15095

The protein tyrosine phosphatase SHP-1 modulates the suppressive activity of regulatory T cells.

Iype T, Sankarshanan M, Mauldin IS, Mullins DW, Lorenz U
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 15;185(10):6115-27

Integrative analysis of epigenetic modulation in melanoma cell response to decitabine: clinical implications.

Halaban R, Krauthammer M, Pelizzola M, Cheng E, Kovacs D, Sznol M, Ariyan S, Narayan D, Bacchiocchi A, Molinaro A, Kluger Y, Deng M, Tran N, Zhang W, Picardo M, Enghild JJ
PloS one 2009;4(2):e4563

Localization of Src homology 2 domain-containing phosphatase 1 (SHP-1) to lipid rafts in T lymphocytes: functional implications and a role for the SHP-1 carboxyl terminus.

Fawcett VC, Lorenz U
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):2849-59

Deficiency of the Src homology region 2 domain-containing phosphatase 1 (SHP-1) causes enrichment of CD4+CD25+ regulatory T cells.

Carter JD, Calabrese GM, Naganuma M, Lorenz U
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):6627-38

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of SHP-1 using SHP-1 Monoclonal Antibody (Product # MA5-11669) on Jurkat Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), Hel 92.1.7 (Lane 2) and KARPAS 299 (Lane 3). The blot was probed with Anti-SHP-1 Mouse monoclonal Antibody (Product # MA5-11669, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 67 kDa band corresponding to SHP-1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE&reg ; 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SHP-1 Monoclonal Antibody (1SH01 (11D7C8H5))(Product # MA5-11669) and a 63kDa band corresponding to SHP-1 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), THP-1 (Lane 2), HEL 92.1.7 (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), U-2 OS (Lane 6), Mouse Spleen (Lane 7), Rat Spleen (Lane 8), Mouse Skeletal Muscle (Lane 9) and Rat Skeletal Muscle (Lane 10), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).The bands observed at 25 kDa are light chain IgG bands in the mouse tissue lysates.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SHP-1 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SHP-1 Mouse Monoclonal Antibody (Product # MA5-11669) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

MA5-11669

Antibody data