Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-32179 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32179, RRID:AB_2173687
- Product name
- Rabbit Polyclonal SHP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PTPN6 Antibody [NBP1-32179] - A. 50 ug rat liver extract 7.5% SDS-PAGE SHP1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PTPN6 Antibody [NBP1-32179] - Sample (30 ug of whole cell lysate) A:NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PTPN6 Antibody [NBP1-32179] - Sample(30 ug of whole cell lysate)A:HeLaS3B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PTPN6 Antibody [NBP1-32179] - HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of SHP1 protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of SHP1 by 3 ug of SHP1 7.5% SDS-PAGE The immunoprecipitated SHP1 protein was detected by SHP1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PTPN6 Antibody [NBP1-32179] - Paraffin-embedded OVCA xenograft, using antibody at 1:100 dilution.