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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- RQ7028 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PTP1C Antibody / PTPN6 / SHP-1
- Antibody type
- Monoclonal
- Description
- This highly specific PTP1C antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 8H11B10
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PTP1C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Jurkat, 2) human Raji, 3) human HeLa, 4) human HepG2, 5) rat RH35 and 6) mouse HEPA1-6 cell lysate with PTP1C antibody. Predicted molecular weight: ~68 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A549 cells with PTP1C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with PTP1C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human renal clear cell carcinoma tissue with PTP1C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human serous adenocarcinoma of the ovary tissue with PTP1C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human smooth muscle fatty carcinoma of the left kidney tissue with PTP1C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Caco-2 cells with PTP1C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PTP1C antibody.
