Antibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF2535 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal MMR/CD206/Mannose Receptor Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant human MMR is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Iron nanoparticle-labeled murine mesenchymal stromal cells in an osteoarthritic model persists and suggests anti-inflammatory mechanism of action.
STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice.
Microglial VPS35 deficiency regulates microglial polarization and decreases ischemic stroke-induced damage in the cortex.
Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury.
Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance.
Skewed macrophage polarization in aging skeletal muscle.
Wnt canonical pathway activator TWS119 drives microglial anti-inflammatory activation and facilitates neurological recovery following experimental stroke.
Transiently proliferating perivascular microglia harbor M1 type and precede cerebrovascular changes in a chronic hypertension model.
Microglial TLR4-dependent autophagy induces ischemic white matter damage via STAT1/6 pathway.
Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice.
Intranasal Stem Cell Treatment as a Novel Therapy for Subarachnoid Hemorrhage.
2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex.
Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury.
B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model.
Serelaxin induces Notch1 signaling and alleviates hepatocellular damage in orthotopic liver transplantation.
Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia.
ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury.
Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment.
Targeting mannose receptor expression on macrophages in atherosclerotic plaques of apolipoprotein E-knockout mice using 111In-tilmanocept.
HDAC3 inhibition ameliorates spinal cord injury by immunomodulation.
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice.
Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury.
Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3β.
miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair.
Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury.
Expression of the homeobox gene HOXA9 in ovarian cancer induces peritoneal macrophages to acquire an M2 tumor-promoting phenotype.
Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4.
MicroRNA-124 as a novel treatment for persistent hyperalgesia.
p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia.
Hamilton AM, Cheung WY, Gómez-Aristizábal A, Sharma A, Nakamura S, Chaboureau A, Bhatt S, Rabani R, Kapoor M, Foster PJ, Viswanathan S
PloS one 2019;14(12):e0214107
PloS one 2019;14(12):e0214107
STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice.
Cai W, Dai X, Chen J, Zhao J, Xu M, Zhang L, Yang B, Zhang W, Rocha M, Nakao T, Kofler J, Shi Y, Stetler RA, Hu X, Chen J
JCI insight 2019 Oct 17;4(20)
JCI insight 2019 Oct 17;4(20)
Microglial VPS35 deficiency regulates microglial polarization and decreases ischemic stroke-induced damage in the cortex.
Ye SY, Apple JE, Ren X, Tang FL, Yao LL, Wang YG, Mei L, Zhou YG, Xiong WC
Journal of neuroinflammation 2019 Nov 26;16(1):235
Journal of neuroinflammation 2019 Nov 26;16(1):235
Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury.
Bellver-Landete V, Bretheau F, Mailhot B, Vallières N, Lessard M, Janelle ME, Vernoux N, Tremblay MÈ, Fuehrmann T, Shoichet MS, Lacroix S
Nature communications 2019 Jan 31;10(1):518
Nature communications 2019 Jan 31;10(1):518
Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance.
Morita-Takemura S, Nakahara K, Hasegawa-Ishii S, Isonishi A, Tatsumi K, Okuda H, Tanaka T, Kitabatake M, Ito T, Wanaka A
Journal of neuroinflammation 2019 Feb 14;16(1):39
Journal of neuroinflammation 2019 Feb 14;16(1):39
Skewed macrophage polarization in aging skeletal muscle.
Cui CY, Driscoll RK, Piao Y, Chia CW, Gorospe M, Ferrucci L
Aging cell 2019 Dec;18(6):e13032
Aging cell 2019 Dec;18(6):e13032
Wnt canonical pathway activator TWS119 drives microglial anti-inflammatory activation and facilitates neurological recovery following experimental stroke.
Song D, Zhang X, Chen J, Liu X, Xue J, Zhang L, Lan X
Journal of neuroinflammation 2019 Dec 6;16(1):256
Journal of neuroinflammation 2019 Dec 6;16(1):256
Transiently proliferating perivascular microglia harbor M1 type and precede cerebrovascular changes in a chronic hypertension model.
Koizumi T, Taguchi K, Mizuta I, Toba H, Ohigashi M, Onishi O, Ikoma K, Miyata S, Nakata T, Tanaka M, Foulquier S, Steinbusch HWM, Mizuno T
Journal of neuroinflammation 2019 Apr 10;16(1):79
Journal of neuroinflammation 2019 Apr 10;16(1):79
Microglial TLR4-dependent autophagy induces ischemic white matter damage via STAT1/6 pathway.
Qin C, Liu Q, Hu ZW, Zhou LQ, Shang K, Bosco DB, Wu LJ, Tian DS, Wang W
Theranostics 2018;8(19):5434-5451
Theranostics 2018;8(19):5434-5451
Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice.
Ye J, Huang Y, Que B, Chang C, Liu W, Hu H, Liu L, Shi Y, Wang Y, Wang M, Zeng T, Zhen W, Xu Y, Shi L, Liu J, Jiang H, Ye D, Lin Y, Wan J, Ji Q
EBioMedicine 2018 Sep;35:29-39
EBioMedicine 2018 Sep;35:29-39
Intranasal Stem Cell Treatment as a Novel Therapy for Subarachnoid Hemorrhage.
Nijboer CH, Kooijman E, van Velthoven CT, van Tilborg E, Tiebosch IA, Eijkelkamp N, Dijkhuizen RM, Kesecioglu J, Heijnen CJ
Stem cells and development 2018 Mar 1;27(5):313-325
Stem cells and development 2018 Mar 1;27(5):313-325
2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex.
Hashimoto K, Nakashima M, Hamano A, Gotoh M, Ikeshima-Kataoka H, Murakami-Murofushi K, Miyamoto Y
Scientific reports 2018 Jun 26;8(1):9715
Scientific reports 2018 Jun 26;8(1):9715
Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury.
Lindborg JA, Niemi JP, Howarth MA, Liu KW, Moore CZ, Mahajan D, Zigmond RE
Journal of neuroinflammation 2018 Jun 26;15(1):192
Journal of neuroinflammation 2018 Jun 26;15(1):192
B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model.
Liu G, Lu P, Chen L, Zhang W, Wang M, Li D, Zhang X
Eye (London, England) 2018 Jul;32(7):1220-1231
Eye (London, England) 2018 Jul;32(7):1220-1231
Serelaxin induces Notch1 signaling and alleviates hepatocellular damage in orthotopic liver transplantation.
Kageyama S, Nakamura K, Ke B, Busuttil RW, Kupiec-Weglinski JW
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2018 Jul;18(7):1755-1763
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2018 Jul;18(7):1755-1763
Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia.
Liu X, Wen S, Yan F, Liu K, Liu L, Wang L, Zhao S, Ji X
Journal of neuroinflammation 2018 Feb 9;15(1):39
Journal of neuroinflammation 2018 Feb 9;15(1):39
ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury.
Yang Y, Liu H, Zhang H, Ye Q, Wang J, Yang B, Mao L, Zhu W, Leak RK, Xiao B, Lu B, Chen J, Hu X
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 May 3;37(18):4692-4704
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 May 3;37(18):4692-4704
Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment.
Gensel JC, Kopper TJ, Zhang B, Orr MB, Bailey WM
Scientific reports 2017 Jan 6;7:40144
Scientific reports 2017 Jan 6;7:40144
Targeting mannose receptor expression on macrophages in atherosclerotic plaques of apolipoprotein E-knockout mice using 111In-tilmanocept.
Varasteh Z, Hyafil F, Anizan N, Diallo D, Aid-Launais R, Mohanta S, Li Y, Braeuer M, Steiger K, Vigne J, Qin Z, Nekolla SG, Fabre JE, Döring Y, Le Guludec D, Habenicht A, Vera DR, Schwaiger M
EJNMMI research 2017 Dec;7(1):40
EJNMMI research 2017 Dec;7(1):40
HDAC3 inhibition ameliorates spinal cord injury by immunomodulation.
Kuboyama T, Wahane S, Huang Y, Zhou X, Wong JK, Koemeter-Cox A, Martini M, Friedel RH, Zou H
Scientific reports 2017 Aug 17;7(1):8641
Scientific reports 2017 Aug 17;7(1):8641
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice.
Sung SJ, Ge Y, Dai C, Wang H, Fu SM, Sharma R, Hahn YS, Yu J, Le TH, Okusa MD, Bolton WK, Lawler JR
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2589-2601
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2589-2601
Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury.
Hellström Erkenstam N, Smith PL, Fleiss B, Nair S, Svedin P, Wang W, Boström M, Gressens P, Hagberg H, Brown KL, Sävman K, Mallard C
Frontiers in cellular neuroscience 2016;10:286
Frontiers in cellular neuroscience 2016;10:286
Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3β.
Jiang C, Zhu W, Yan X, Shao Q, Xu B, Zhang M, Gong R
Scientific reports 2016 Nov 18;6:36698
Scientific reports 2016 Nov 18;6:36698
miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair.
Gaudet AD, Mandrekar-Colucci S, Hall JC, Sweet DR, Schmitt PJ, Xu X, Guan Z, Mo X, Guerau-de-Arellano M, Popovich PG
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 10;36(32):8516-32
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 10;36(32):8516-32
Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
Kan MJ, Lee JE, Wilson JG, Everhart AL, Brown CM, Hoofnagle AN, Jansen M, Vitek MP, Gunn MD, Colton CA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 15;35(15):5969-82
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 15;35(15):5969-82
Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury.
Wang J, Xia J, Zhang F, Shi Y, Wu Y, Pu H, Liou AK, Leak RK, Yu X, Chen L, Chen J
Scientific reports 2015 Apr 10;5:9621
Scientific reports 2015 Apr 10;5:9621
Expression of the homeobox gene HOXA9 in ovarian cancer induces peritoneal macrophages to acquire an M2 tumor-promoting phenotype.
Ko SY, Ladanyi A, Lengyel E, Naora H
The American journal of pathology 2014 Jan;184(1):271-81
The American journal of pathology 2014 Jan;184(1):271-81
Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4.
Pepe G, Calderazzi G, De Maglie M, Villa AM, Vegeto E
Journal of neuroinflammation 2014 Dec 31;11:211
Journal of neuroinflammation 2014 Dec 31;11:211
MicroRNA-124 as a novel treatment for persistent hyperalgesia.
Willemen HL, Huo XJ, Mao-Ying QL, Zijlstra J, Heijnen CJ, Kavelaars A
Journal of neuroinflammation 2012 Jun 25;9:143
Journal of neuroinflammation 2012 Jun 25;9:143
p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia.
Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH
The American journal of pathology 2009 Jan;174(1):153-63
The American journal of pathology 2009 Jan;174(1):153-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse MMR/CD206 by Western Blot. Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- MMR/CD206 in Mouse Testis. MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- MMR/CD206 in Mouse Lung. MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.