MA5-32498
antibody from Invitrogen Antibodies
Targeting: MRC1
bA541I19.1, CD206, CLEC13D, CLEC13DL, MRC1L1
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
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Validation data
Reference
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- Product number
- MA5-32498 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD206 (MMR) Recombinant Rabbit Monoclonal Antibody (JF0953)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JF0953
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Data-Driven Kidney Transplant Phenotyping as a Histology-Independent Framework for Biomarker Discovery.
Buscher K, Heitplatz B, van Marck V, Song J, Loismann S, Rixen R, Hüchtmann B, Kurian S, Ehinger E, Wolf D, Ley K, Pavenstädt H, Reuter S
Journal of the American Society of Nephrology : JASN 2021 Aug;32(8):1933-1945
Journal of the American Society of Nephrology : JASN 2021 Aug;32(8):1933-1945
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD206 (MMR) in different lysates using a Monoclonal antibody (Product #MA5-32498) at a dilution of 1:1,000. Positive control: Lane 1: Human lung, Lane 2: HepG2, Lane 3: 293T.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD206 (MMR) in different lysates using a Monoclonal antibody (Product #MA5-32498) at a dilution of 1:1,000. Positive control: Lane 1: Human lung, Lane 2: HepG2, Lane 3: 293T.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Macrophage mannose receptor 1 was achieved by transfecting MOLT-4 with Macrophage mannose receptor 1 specific siRNAs (Silencer® select Product # S8956, S8958). Western Blot analysis (Fig. a) was performed using Membrane enriched extracts from the Macrophage mannose receptor 1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with CD206 (MMR) Recombinant Rabbit Monoclonal Antibody (JF0953) (Product # MA5-32498, 1:1000 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western Blot is shown in histogram (Fig. b). A decrease in signal upon siRNA mediated knockdown confirms that antibody is specific to Macrophage mannose receptor 1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-CD206 (MMR) Recombinant Rabbit Monoclonal Antibody (JF0953) (Product # MA5-32498) and a 160 kDa band corresponding to Macrophage mannose receptor 1 was observed across cell lines tested except NIH:OVCAR-3 which is reported to be low for CD206 expression. Membrane enriched extracts (30 µg lysate) of Reh (Lane 1), NIH:OVCAR-3 (Lane 2) and MOLT-4 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CD206 (MMR) in Hela cells using a CD206 (MMR) Monoclonal antibody (Product # MA5-32498) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CD206 (MMR) in MCF-7 cells using a CD206 (MMR) Monoclonal antibody (Product # MA5-32498) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CD206 (MMR) in HepG2 cells using a CD206 (MMR) Monoclonal antibody (Product # MA5-32498) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.