A301-224A

antibody from Invitrogen Antibodies

Targeting: CHD8 DUPLIN, HELSNF1, KIAA1564

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-224A

Provider

Invitrogen Antibodies

Product name

CHD8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD8 antibody (Product # A301-224A lot 3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody A301-224A (lot A301-224A-2) used for IP at 6 µg/mg lysate. CHD8 was also immunoprecipitated by rabbit anti-CHD8 antibody A301-225A. For blotting immunoprecipitated CHD8, A301-224A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse CHD8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody A301-224A (lot A301-224A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHD8 (Product # A301-224A; lot 3) used at a dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 (Cat. No. A301-224A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHD8 antibody (Product # A301-224A lot 3) used for IP at 6 µg per reaction. CHD8 was also immunoprecipitated by a previous lot of this antibody (A301-224A lot 2) and another antibody against a different epitope of CHD8 (Product # A301-225A). For blotting immunoprecipitated CHD8 (Product # A301-224A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.