A301-224A

antibody from Invitrogen Antibodies

Targeting: CHD8 DUPLIN, HELSNF1, KIAA1564

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-224A

Provider

Invitrogen Antibodies

Product name

CHD8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD8 antibody (Product # A301-224A lot 3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody A301-224A (lot A301-224A-2) used for IP at 6 µg/mg lysate. CHD8 was also immunoprecipitated by rabbit anti-CHD8 antibody A301-225A. For blotting immunoprecipitated CHD8, A301-224A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse CHD8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody A301-224A (lot A301-224A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHD8 (Product # A301-224A; lot 3) used at a dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 (Cat. No. A301-224A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHD8 antibody (Product # A301-224A lot 3) used for IP at 6 µg per reaction. CHD8 was also immunoprecipitated by a previous lot of this antibody (A301-224A lot 2) and another antibody against a different epitope of CHD8 (Product # A301-225A). For blotting immunoprecipitated CHD8 (Product # A301-224A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.