Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-77293 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAI1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAI1 in rat brain lysates (lane 1), mouse brain lysates (lane 2), rat brain lysates preincubated with antigen (lane 3), mouse brain lysates preincubated with antigen (lane 4). Samples were probed using a BAI1 (extracellular) polyclonal antibody (Product # PA5-77293) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAI1 in rat brain lysates (lane 1), mouse brain lysates (lane 2), rat brain lysates preincubated with antigen (lane 3), mouse brain lysates preincubated with antigen (lane 4). Samples were probed using a BAI1 (extracellular) polyclonal antibody (Product # PA5-77293) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAI1 in human HL-60 promyelocytic leukemia cell lysates (lanes 1 and 2. Lane 2 was preincubated with antigen) using BAI1 (extracellular) polyclonal antibody (Product # PA5-77293) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BAI1 (extracellular) in mouse olfactory bulb. A) Samples were probed with BAI1 (extracellular) polyclonal antibody (Product # PA5-77293) using a dilution of 1:200, and incubated with GFAP and DAPI. B) Double-staining of BAI1 (green) and glial fibrillary acidic protein (red) reveals expression of BAI1 in a subset of astrocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of human HL-60 promyelocytic leukemia cells using polyclonal BAI1 (extracellular) antibody (Product # PA5-77293) with a dilution of 1:20 followed by a goat-anti-rabbit-AlexaFluor-488 secondary antibody.