PA1-26702

antibody from Invitrogen Antibodies

Targeting: LCK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-26702

Provider

Invitrogen Antibodies

Product name

Anti-Phospho-LCK (Tyr192) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-26702 detects Lck (phospho Y192) from human samples. PA1-26702 is expected to cross react with chicken (79% conserved) and mouse (100% conserved) due to sequence homology. PA1-26702 has been successfully used in Western blot procedures. The PA1-26702 immunogen is a synthetic phosphopeptide corresponding to a portion of human Lck containing tyrosine 192. The sequence is conserved in human and mouse.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.5 mg/ml

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Background extracts alone (1) or with added recombinant active human Lck protein (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, and then incubated with a Phospho-Lck (pTyr192) antibody (Product # PA1-26702) in a 1% BSA-TBST buffer for two hours at room temperature, following incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4) or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F (ab)2 anti-rabbit IgG HRP conjugated secondary antibody. The data show that only the phosphopeptide corresponding to Lck (pTrY192) blocks the antibody signal, demonstrating the specificity of the antibody.