H00003932-M11

antibody from Abnova Corporation

Targeting: LCK

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003932-M11

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003932-M11, RRID:AB_1137281

Product name

LCK monoclonal antibody (M11), clone 3H5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant LCK.

Antigen sequence

MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGT
LLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALH
SYEPSHDGDLGFEKGEQLRILEQSGEWWKA


Isotype

IgG

Antibody clone number

3H5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody (M11), clone 3H5.Lane 1: LCK transfected lysate(58.001 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged LCK is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol