H00003932-D01P

antibody from Abnova Corporation

Targeting: LCK

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003932-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003932-D01P, RRID:AB_1677327

Product name

LCK purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human LCK protein.

Antigen sequence

MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGT
LLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALH
SYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTT
GQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQ
LLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQG
EVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYT
NASDGLCTRLSRPCQTQKPQKPWWEDEWEVPRETL
KLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSM
SPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYII
TEYMENDTLLDSQLEEKGLGASPWGNLGQQLLLLP
TGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFI
EERNYIHRDLRAANILVSDTLSCKIADFGLARLIE
DNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWS
FGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMV
RPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLE
DFFTATEGQYQPQP


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

LCK MaxPab rabbit polyclonal antibody. Western Blot analysis of LCK expression in Jurkat.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of LCK expression in transfected 293T cell line (H00003932-T02) by LCK MaxPab polyclonal antibody.Lane 1: LCK transfected lysate(61.20 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between LCK and CD55. HeLa cells were stained with anti-LCK rabbit purified polyclonal 1:1200 and anti-CD55 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)