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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- 50-9076-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-LCK (Tyr505) Monoclonal Antibody (SRRCHA), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SRRCHA
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Feedback circuits monitor and adjust basal Lck-dependent events in T cell receptor signaling.
Function of the Src-family kinases, Lck and Fyn, in T-cell development and activation.
Regulation of Lck activity by CD4 and CD28 in the immunological synapse.
Schoenborn JR, Tan YX, Zhang C, Shokat KM, Weiss A
Science signaling 2011 Sep 13;4(190):ra59
Science signaling 2011 Sep 13;4(190):ra59
Function of the Src-family kinases, Lck and Fyn, in T-cell development and activation.
Palacios EH, Weiss A
Oncogene 2004 Oct 18;23(48):7990-8000
Oncogene 2004 Oct 18;23(48):7990-8000
Regulation of Lck activity by CD4 and CD28 in the immunological synapse.
Holdorf AD, Lee KH, Burack WR, Allen PM, Shaw AS
Nature immunology 2002 Mar;3(3):259-64
Nature immunology 2002 Mar;3(3):259-64
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TOP: Intracellular staining of untreated (left) or 5-minute hydrogen peroxide-activated sodium pervanadate-treated (right) normal human peripheral blood cells with Atni-Human CD3 PE (Product # 12-0037-42) and Anti-Human/Mouse phospho-LCK (Y505) eFluor® 660, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocytes gate were used for analysis. BOTTOM: Intracellular staining of untreated (orange histogram) or 5-minute hydrogen peroxide-activated sodium pervanadate-treated (purple histogram) normal human peripheral blood cells with Anti-Human CD3 PE (Product # 12-0037-42), Anti-Human CD19 FITC (Product # 11-0199-42), and Anti-Human/Mouse phospho-LCK (Y505) eFluor® 660, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Lymphocytes in the CD3+ (left) or CD19+ (right) gates were used for analysis.
