Antibody data

Product number
Ab112898 - Provider product page
Provider
Aladdin Scientific
Product name
Anti-Lck antibody
Antibody type
Recombinant
Description
Rabbit anti Human Lck Antibody, Recombinant (R07-5M3), could be used for WB and so on.Application:WB: 1/500-1/1000Protein FunctionTyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.
Reactivity
Human
Host
Rabbit
Conjugate
Unconjugated
Antigen sequence
AA 21-75
Epitope
reacts with an epitope located in the region before SH3 domain of Lck.
Antibody clone number
R07-5M3
Vial size
100μl,10μl,1ml,50μl
Concentration
0,5 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
LCK protein structure - Ab112898 shown in red.