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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- NBP2-29631 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD45RO Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) [NBP2-29631] - Formalin-fixed, paraffin-embedded human tonsil stained with CD45RO antibody (1:500), peroxidase-conjugate and DAB chromogen. Note cell membrane staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) [NBP2-29631] - Staining of human tonsil
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631] - CD45RO expression by lymphocyte gated population of PBMC: PBMC were stained either with purified CD45RO antibody (0.5 ug/10^6 cells) or isotype control following surface staining protocol. PE-conjugated goat anti-mouse IgG, secondary antibody used to identify the positive staining on PPI negative lymphocyte gated population.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45RO Antibody (UCHL-1) [NBP2-29631] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Antibody (UCHL-1) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
