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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP1-43408 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-43408, RRID:AB_10006849
- Product name
- Mouse Monoclonal B220/CD45R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: B220/CD45R Antibody (HIS24) [NBP1-43408] - Staining of rat splenocytes with 0.5 ug of Mouse IgG2b K Isotype Control FITC (blue histogram) or 0.5 ug of Anti-Rat CD45R (B220) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis (left). Immunohistochemistry of frozen rat spleen using 10 ug/mL Anti-Rat CD45R (B220) FITC (green), 10 ug/mL Anti-Rat Marginal Zone B Cells eFluor 660 (white), and 10 ug/mL Anti-Rat CD3 Biotin followed by 1 ug/mL Streptavidin eFluor (R) 570 (red). Nuclei are stained with DAPI (blue)(right).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408] - Analysis using the FITC conjugate of NBP1-43408. Staining of rat splenocytes with 0.05 ug of Mouse IgG2b K Isotype Control FITC (open histogram) or 0.05 ug of Anti-Rat CD45R (B220) FITC (filled histogram). Total viable cells were used for analysis.
