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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- NBP2-15811 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal CD45 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Characterization of Apoptosis, Autophagy and Oxidative Stress in Pancreatic Islets Cells and Intestinal Epithelial Cells Isolated from Equine Metabolic Syndrome (EMS) Horses.
Validation of Flow Cytometry and Magnetic Bead-Based Methods to Enrich CNS Single Cell Suspensions for Quiescent Microglia.
Kornicka K, Śmieszek A, Szłapka-Kosarzewska J, Irwin Houston JM, Roecken M, Marycz K
International journal of molecular sciences 2018 Oct 8;19(10)
International journal of molecular sciences 2018 Oct 8;19(10)
Validation of Flow Cytometry and Magnetic Bead-Based Methods to Enrich CNS Single Cell Suspensions for Quiescent Microglia.
Volden TA, Reyelts CD, Hoke TA, Arikkath J, Bonasera SJ
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2015 Dec;10(4):655-65
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2015 Dec;10(4):655-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD45 Antibody [NBP2-15811] - Raji whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody [NBP2-15811] - Mouse splenocytes cell fixed in 4% paraformaldehyde at 4 C for 15 min. Black: Unlabelled sample was used as a control. Red: CD45 antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488- Conjugated secondary antibody for FACS analysis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody [NBP2-15811] - THP-1 cell. Black: Unlabelled sample was used as a control.Red: CD45 antibody (GTX116018) dilution: 1:50.
