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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [25]
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- Validations
- Flow cytometry [3]
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- Product number
- NB100-64895 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64895, RRID:AB_960392
- Product name
- Mouse Monoclonal CD45 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.25 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Attenuating Neurogenic Sympathetic Hyperreflexia Robustly Improves Antibacterial Immunity After Chronic Spinal Cord Injury.
Trafficking of immune cells across the blood-brain barrier is modulated by neurofibrillary pathology in tauopathies.
Washing or filtering of blood products does not improve outcome in a rat model of trauma and multiple transfusion.
Should platelet-rich plasma be activated in fat grafts? An animal study.
Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity.
Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity.
Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.
Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.
Differential effects of oral versus intrathymic administration of polymorphic major histocompatibility complex class II peptides on mononuclear and endothelial cell activation and cytokine expression during a delayed-type hypersensitivity response.
Mesothelial cell injury caused by pleural leukocytes from rats treated with intratracheal instillation of crocidolite asbestos or Corynebacterium parvum.
Functional and phenotypical analysis of subsets of rat CD4+ T cells.
Functional and phenotypical analysis of subsets of rat CD4+ T cells.
Molecular associations between the T-lymphocyte antigen receptor complex and the surface antigens CD2, CD4, or CD8 and CD5.
Molecular associations between the T-lymphocyte antigen receptor complex and the surface antigens CD2, CD4, or CD8 and CD5.
Inhibition of T cell responses in vitro by an antibody against a novel lymphocyte surface molecule (QCA-1).
Vascular thymus transplantation in rats. Technique, morphology, and function.
Vascular thymus transplantation in rats. Technique, morphology, and function.
RT7-defined alloantigens in rats are part of the leucocyte common antigen family.
T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.
T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.
Interstitial nephritis induced by protein-overload proteinuria.
Interleukin 2 receptor in rat heart allograft rejection.
Interleukin 2 receptor in rat heart allograft rejection.
Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.
Mironets E, Fischer R, Bracchi-Ricard V, Saltos TM, Truglio TS, O'Reilly ML, Swanson KA, Bethea JR, Tom VJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jan 8;40(2):478-492
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jan 8;40(2):478-492
Trafficking of immune cells across the blood-brain barrier is modulated by neurofibrillary pathology in tauopathies.
Majerova P, Michalicova A, Cente M, Hanes J, Vegh J, Kittel A, Kosikova N, Cigankova V, Mihaljevic S, Jadhav S, Kovac A
PloS one 2019;14(5):e0217216
PloS one 2019;14(5):e0217216
Washing or filtering of blood products does not improve outcome in a rat model of trauma and multiple transfusion.
Wirtz MR, Jurgens J, Zuurbier CJ, Roelofs JJTH, Spinella PC, Muszynski JA, Carel Goslings J, Juffermans NP
Transfusion 2019 Jan;59(1):134-145
Transfusion 2019 Jan;59(1):134-145
Should platelet-rich plasma be activated in fat grafts? An animal study.
Hersant B, Bouhassira J, SidAhmed-Mezi M, Vidal L, Keophiphath M, Chheangsun B, Niddam J, Bosc R, Nezet AL, Meningaud JP, Rodriguez AM
Journal of plastic, reconstructive & aesthetic surgery : JPRAS 2018 May;71(5):681-690
Journal of plastic, reconstructive & aesthetic surgery : JPRAS 2018 May;71(5):681-690
Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity.
Stumbles PA, Thomas JA, Pimm CL, Lee PT, Venaille TJ, Proksch S, Holt PG
The Journal of experimental medicine 1998 Dec 7;188(11):2019-31
The Journal of experimental medicine 1998 Dec 7;188(11):2019-31
Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity.
Stumbles PA, Thomas JA, Pimm CL, Lee PT, Venaille TJ, Proksch S, Holt PG
The Journal of experimental medicine 1998 Dec 7;188(11):2019-31
The Journal of experimental medicine 1998 Dec 7;188(11):2019-31
Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.
Crook K, Hunt SV
Developmental immunology 1996;4(4):235-46
Developmental immunology 1996;4(4):235-46
Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.
Crook K, Hunt SV
Developmental immunology 1996;4(4):235-46
Developmental immunology 1996;4(4):235-46
Differential effects of oral versus intrathymic administration of polymorphic major histocompatibility complex class II peptides on mononuclear and endothelial cell activation and cytokine expression during a delayed-type hypersensitivity response.
Hancock WW, Khoury SJ, Carpenter CB, Sayegh MH
The American journal of pathology 1994 Jun;144(6):1149-58
The American journal of pathology 1994 Jun;144(6):1149-58
Mesothelial cell injury caused by pleural leukocytes from rats treated with intratracheal instillation of crocidolite asbestos or Corynebacterium parvum.
Li XY, Lamb D, Donaldson K
Environmental research 1994 Feb;64(2):181-91
Environmental research 1994 Feb;64(2):181-91
Functional and phenotypical analysis of subsets of rat CD4+ T cells.
Nagai Y, Inobe M, Kikuchi K, Uede T
Microbiology and immunology 1993;37(8):623-32
Microbiology and immunology 1993;37(8):623-32
Functional and phenotypical analysis of subsets of rat CD4+ T cells.
Nagai Y, Inobe M, Kikuchi K, Uede T
Microbiology and immunology 1993;37(8):623-32
Microbiology and immunology 1993;37(8):623-32
Molecular associations between the T-lymphocyte antigen receptor complex and the surface antigens CD2, CD4, or CD8 and CD5.
Beyers AD, Spruyt LL, Williams AF
Proceedings of the National Academy of Sciences of the United States of America 1992 Apr 1;89(7):2945-9
Proceedings of the National Academy of Sciences of the United States of America 1992 Apr 1;89(7):2945-9
Molecular associations between the T-lymphocyte antigen receptor complex and the surface antigens CD2, CD4, or CD8 and CD5.
Beyers AD, Spruyt LL, Williams AF
Proceedings of the National Academy of Sciences of the United States of America 1992 Apr 1;89(7):2945-9
Proceedings of the National Academy of Sciences of the United States of America 1992 Apr 1;89(7):2945-9
Inhibition of T cell responses in vitro by an antibody against a novel lymphocyte surface molecule (QCA-1).
Kampinga J, Kroese FG, Pol GH, Meedendorp B, van Eendenburg J, Groen H, van den Bogaerde J, Nieuwenhuis P, Roser B, Aspinall R
International immunology 1990;2(10):915-20
International immunology 1990;2(10):915-20
Vascular thymus transplantation in rats. Technique, morphology, and function.
Kampinga J, Schuurman HJ, Pol GH, Bartels H, Broekhuizen R, Vaessen LM, Tielen FJ, Rozing J, Blaauw EH, Roser B
Transplantation 1990 Oct;50(4):669-78
Transplantation 1990 Oct;50(4):669-78
Vascular thymus transplantation in rats. Technique, morphology, and function.
Kampinga J, Schuurman HJ, Pol GH, Bartels H, Broekhuizen R, Vaessen LM, Tielen FJ, Rozing J, Blaauw EH, Roser B
Transplantation 1990 Oct;50(4):669-78
Transplantation 1990 Oct;50(4):669-78
RT7-defined alloantigens in rats are part of the leucocyte common antigen family.
Kampinga J, Kroese FG, Pol GH, Opstelten D, Seijen HG, Boot JH, Roser B, Nieuwenhuis P, Aspinall R
Scandinavian journal of immunology 1990 Jun;31(6):699-710
Scandinavian journal of immunology 1990 Jun;31(6):699-710
T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.
Lazo PA, Klein-Szanto AJ, Tsichlis PN
Journal of virology 1990 Aug;64(8):3948-59
Journal of virology 1990 Aug;64(8):3948-59
T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.
Lazo PA, Klein-Szanto AJ, Tsichlis PN
Journal of virology 1990 Aug;64(8):3948-59
Journal of virology 1990 Aug;64(8):3948-59
Interstitial nephritis induced by protein-overload proteinuria.
Eddy AA
The American journal of pathology 1989 Oct;135(4):719-33
The American journal of pathology 1989 Oct;135(4):719-33
Interleukin 2 receptor in rat heart allograft rejection.
Bouchot O, Anegon I, Romaniuk A, Jacques Y, Paineau J, Soulillou JP
Transplantation 1989 Dec;48(6):918-22
Transplantation 1989 Dec;48(6):918-22
Interleukin 2 receptor in rat heart allograft rejection.
Bouchot O, Anegon I, Romaniuk A, Jacques Y, Paineau J, Soulillou JP
Transplantation 1989 Dec;48(6):918-22
Transplantation 1989 Dec;48(6):918-22
Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
Woollett GR, Barclay AN, Puklavec M, Williams AF
European journal of immunology 1985 Feb;15(2):168-73
European journal of immunology 1985 Feb;15(2):168-73
Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.
Sunderland CA, McMaster WR, Williams AF
European journal of immunology 1979 Feb;9(2):155-9
European journal of immunology 1979 Feb;9(2):155-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Staining of rat peripheral blood lymphocytes with mouse anti-rat CD45 OX-1 clone.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 OX-1 clone. Rat Splenocytes were stained with CD45 (OX-1) antibody 100-64895 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using the FITC conjugate of CD45 OX-1 clone. Staining of rat spleen cells.
